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Showing: 961 - 980 of 1639 results

  1. pBBR-Cas9ΔyqhD-HA500

    Plasmid
    #220369
    Purpose
    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440
    Depositor
    Yilan Liu
    Inserts
    SpCas9
    sgRNA for yqhD in P. putida KT2440
    Use
    CRISPR; Broad host range
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    220369-plasmid-map-sequence-id-437258

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  2. pX330-MM002-ZFP143-C-terminal-sgRNA

    Plasmid
    #212706
    Purpose
    Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus
    Depositor
    Elzo de Wit
    Insert
    ZFP143 C-terminal sgRNA (Zfp143 Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    212706-plasmid-map-sequence-id-438797

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  3. pHairyRed (double 35s removed)

    Plasmid
    #69710
    Purpose
    Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots - modified
    Depositor
    Brett Ferguson, Peter Gresshoff
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    69710-plasmid-map-sequence-id-260035

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  4. MSP2133

    Plasmid
    #72249
    Purpose
    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    Y450A, N497A, R661A, Q695A, and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72249-plasmid-map-sequence-id-131869

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  5. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

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  6. pH-STEME-1-esgRNA

    Plasmid
    #138135
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138135-plasmid-map-sequence-id-311741

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  7. pH-STEME-NG-esgRNA

    Plasmid
    #138136
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138136-plasmid-map-sequence-id-311723

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  8. lenti.Cas9.BFP.sgRNA.parental

    Plasmid
    #196715
    Purpose
    Sortable WT-SpCas9 expression and guide cassette (1-vector system).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196715-plasmid-map-sequence-id-389692

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  9. MSP2440

    Plasmid
    #72250
    Purpose
    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72250-plasmid-map-sequence-id-131871

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  10. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121817
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121817-plasmid-map-sequence-id-237365

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  11. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121816
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121816-plasmid-map-sequence-id-237364

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  12. pRR1

    Plasmid
    #166096
    Purpose
    Gal inducible expression of Cas9 WT
    Depositor
    Rebecca Burgess
    Insert
    WT spCas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166096-plasmid-map-sequence-id-330354

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  13. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

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  14. pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)

    Plasmid
    #160634
    Purpose
    TU for wtGFP expression driven by 2x35s promoter.
    Depositor
    Diego Orzaez
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    2x35s
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160634-plasmid-map-sequence-id-321964

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  15. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140002-plasmid-map-sequence-id-275516

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  16. pITI2

    Plasmid
    #166095
    Purpose
    Gal inducible expression of D10A-Cas9 nickase
    Depositor
    Rebecca Burgess
    Insert
    Cas9-D10A
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A
    Promoter
    Gal-inducible
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    166095-plasmid-map-sequence-id-335585

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  17. pPH3

    Plasmid
    #166098
    Purpose
    Gal inducible expression of D10A-Cas9 nickase
    Depositor
    Rebecca Burgess
    Insert
    Cas9-D10A
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A
    Promoter
    Gal-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166098-plasmid-map-sequence-id-330351

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  18. pDGB3 alpha1 P35S:BFP:T35S (GB3416)

    Plasmid
    #160637
    Purpose
    TU for the constitutive expression of Blue Fluorescent Protein
    Depositor
    Diego Orzaez
    Insert
    BFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160637-plasmid-map-sequence-id-321961

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  19. pEGB3_alpha1 Pnos:P19:Tnos (GB2762)

    Plasmid
    #160632
    Purpose
    TU for expression of P19 driven by Nos promoter.
    Depositor
    Diego Orzaez
    Insert
    P19
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160632-plasmid-map-sequence-id-321966

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  20. pCJ1022

    Plasmid
    #228762
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Pkd2 gRNAs (Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    228762-plasmid-map-sequence-id-455850

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Showing: 961 - 980 of 1639 results