161,697 results

1. pcDNA3.1-HA-RAD52-∆C

   Plasmid

   #235677

   Purpose

   Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Insert

   RAD52 (RAD52 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted amino acids 302-410

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

   Plasmid

   #233035

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

   Depositor

   Jonathan Ploski

   Insert

   RfxCas13d-compatible gRNA expression cassette and CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXJ-His-PDCD1LG2

   Plasmid

   #232660

   Purpose

   transient expresses PD-L2 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   PDCD1LG2 (PDCD1LG2 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AiP12311 - pAAV-AiE0226h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2311)

   Plasmid

   #230108

   Purpose

   AiE0226h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Boaz Levi

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ GFP-2xESP3I-IRES-mCherry

   Plasmid

   #230996

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Type

   Empty backbone

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV-CAG-oScarlet-WPRE-BC5-pA

   Plasmid

   #220769

   Purpose

   Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al bioRxiv 2024.04.24.590975

   Insert

   oScarlert-WPRE-BC5
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV-CAG-oScarlet-WPRE-BC4-pA

   Plasmid

   #220768

   Purpose

   Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al bioRxiv 2024.04.24.590975

   Insert

   oScarlert-WPRE-BC4
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-memRFP-3xControlgRNA

   Plasmid

   #224567

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   CRISPR

   Tags

   Membrane localization signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX

   Plasmid

   #214282

   Purpose

   Express split-RhoA fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
   ras homolog family member A (RHOA Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   RhoA-Q63L

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV-CAG-oScarlet-WPRE-BC6-pA

    Plasmid

    #220770

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC6
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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