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EGFP-Bin1b

Plasmid

#176014

Purpose

Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v8-10 (-cyt)

Plasmid

#137822

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v8-10 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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FUW TetO NET1A-V5 WT

Plasmid

#69828

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-402-0)

Plasmid

#89823

Purpose

Promoter luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Promoter

SV40

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37 SHPmut

Plasmid

#113503

Purpose

Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

F215A/E218A/QKL220-222AAA

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2B

Plasmid

#40039

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2B (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2B domain (488-671)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pMX-IY GBRPL2

Plasmid

#89295

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 hSlp2-a del.C2AB

Plasmid

#40059

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a del.C2AB (SYTL2 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion of C2A and C2B domains (601-910)

Promoter

Tac

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pTH744-CEN-RLuc/slowmaxCFLuc

Plasmid

#38224

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD

Plasmid

#114057

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp2-a V18A

Plasmid

#40054

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a V18A (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Valine 18 to Alanine

Promoter

CMV promoter

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-CARMA2cl-HA

Plasmid

#97195

Purpose

Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 2 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37 deltaUBX

Plasmid

#113504

Purpose

Bacterial expression of GST-tagged p37 lacking the UBX domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 251-331 (stop codon behind AA250)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST NET1A S46A

Plasmid

#69818

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Tags

V5

Expression

Mammalian

Mutation

S46A

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 Slp2-a C2AB

Plasmid

#40058

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a C2AB (Sytl2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C2AB domains only

Promoter

Tac

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 Slp2-a C2B

Plasmid

#40053

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a C2B (Sytl2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

C2B domain

Promoter

CMV promoter

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pSLIK-Neo-TmiR-Gb2

Plasmid

#25744

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG mFoxg1-GFP T271E

Plasmid

#73057

Purpose

C-terminal GFP fusion of Mouse Foxg1 T271E

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

Foxg1 (Foxg1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

T271E

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG mFoxg1-GFP T271A

Plasmid

#73047

Purpose

C-terminal fusion of gfp Mouse Foxg1 T271A

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

Foxg1 (Foxg1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

T271A

Promoter

CAG

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTH742-CEN-RLuc/slowminCFLuc

Plasmid

#38222

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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