We narrowed to 23,077 results for: ESC

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pFX-mCherry-Rab7

Plasmid

#174458

Purpose

Express mCherry with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Rab11

Plasmid

#174460

Purpose

Express iRFP with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with recycling endosome-targeting sequence

Tags

small GTPase (Rab11)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138131

Purpose

Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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AA177

Plasmid

#215983

Purpose

Fragmid fragment: (N' terminus) Start codon only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA178

Plasmid

#216046

Purpose

Fragmid fragment: (C' terminus) Stop only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Rab5

Plasmid

#174455

Purpose

Express mCherry with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-CreiLOV-P2A-FusionRed

Plasmid

#192818

Purpose

Expresses CreiLOV with FusionRed tag in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

CreiLOV

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Lck-mTurquoise2

Plasmid

#98822

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lck

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-ｍCherry

Plasmid

#174188

Purpose

Express mCherry with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER13 miR-200c-141

Plasmid

#81020

Purpose

Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

Depositor

Jeffrey Rosen, Thomas Westbrook

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

miR-200c (MIR200C Human)

Use

Lentiviral

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry3V(1-10)

Plasmid

#117483

Purpose

Lentiviral vector for constitutive expression of sfCherry3V(1-10)

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3V(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

6 substitutions (K45R, G52D, L83W, T106A, K182R, …

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTD-NTwinStrep_Km

Plasmid

#45938

Purpose

use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

6xHis and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B_HaloTag7

Plasmid

#169329

Purpose

Expression of H2B-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 (H2BC21 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA1-mRuby3

Plasmid

#184888

Purpose

Stable expression of ANXA1-mRuby3 (Annexin A1-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA1 (ANXA1 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMS_366

Plasmid

#182131

Purpose

expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

Depositor

George Church

Article

Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

Inserts

retron RNA
ec.86 RT
CspRecT

Mutation

incorporated rifampicin-resistance-conferring don…

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA4mt

Plasmid

#58220

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA4mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L F395W D436E

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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tdTomato-Mito-7

Plasmid

#58115

Purpose

Localization: Mitochondria, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

Mito (COX8A Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-PM-ExRai-AktAR2

Plasmid

#184050

Purpose

Plasma membrane-targeted ExRai-AktAR2

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

PM-ExRai-AktAR2

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3830

Plasmid

#200038

Purpose

Prig-3 zif-1 SL2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of zif RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ZIF (zif-1 Nematode)

Tags

mCherry

Expression

Worm

Promoter

Prig-3

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93-mut

Plasmid

#87335

Purpose

To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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