We narrowed to 10,125 results for: Uty

1. Venus2-K140R-STAT3

   Plasmid

   #123169

   Purpose

   Expresses K140R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   K140R substitution

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF CAG luc-EGFP-cre puro

   Plasmid

   #67503

   Purpose

   Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   luciferase-EGFP-cre
   

   Use

   Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-mCherry-WT-KRAS-IRES-Blast

   Plasmid

   #153335

   Purpose

   Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cells

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   mCherry-WT-KRAS (KRAS Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GPER-DuET

   Plasmid

   #213256

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPER (GPER1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX313-TP53-P278A

   Plasmid

   #118015

   Purpose

   Constitutive expression of mutant human Tumor Protein p53 (TP53)

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   TP53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   p.Pro278Ala

   Promoter

   EF1alpha

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZ M2rtTA_CAGG TetON-Sox10 with GFP

   Plasmid

   #115241

   Purpose

   RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Inserts

   SOX10 (SOX10 Human)
   eGFP
   

   Tags

   GFP (separate polypeptide)

   Expression

   Mammalian

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry

   Plasmid

   #172640

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_HA.V5.VSVg_NGFR

   Plasmid

   #158244

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.V5.VSVg

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. G protein alpha-q-CFP

   Plasmid

   #66081

   Purpose

   This G protein alpha-q construct contains internal insertions of CFP and the EE epitope

   Depositor

   Catherine Berlot

   Article

   Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

   Insert

   G protein alpha-q-EE-CFP (Gnaq Mouse)
   

   Tags

   CFP was inserted internally into the alpha-q sequ…

   Expression

   Mammalian

   Mutation

   BamHI and SacI sites in alpha-q were removed with…

   Promoter

   CMV

   Available Since

   July 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

    Plasmid

    #160628

    Purpose

    Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S, 35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-K-RAS V12

    Plasmid

    #31200

    Purpose

    Gateway donor vector containing KRAS G12V.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    K-Ras (KRAS Human)
    

    Use

    Gateway donor vector

    Mutation

    G12V constitutively active

    Available Since

    June 24, 2011

    Availability

    Academic Institutions and Nonprofits only

12. PCDH-METTL14-R298P

    Plasmid

    #141113

    Purpose

    Overexpression of METTL14 mutant in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    HMETTL14 (METTL14 Human)
    

    Expression

    Mammalian

    Mutation

    Substitution - Missense, position 298, R➞P

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-FLAG-2xSTREP-CDKN1B-Puro

    Plasmid

    #172611

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDKN1B (CDKN1B Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX_TRC311 ETV5

    Plasmid

    #74984

    Purpose

    Constitutive ETV5 ORF (codon optimized), blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Inserts

    ETV5 (ETV5 Human)
    ETV5 (ETV5 Human)
    

    Expression

    Mammalian

    Mutation

    Codon optimized (synonymous)

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. CXCR4-DuET

    Plasmid

    #213221

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR4 (CXCR4 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-STAT-CA

    Plasmid

    #195576

    Purpose

    Expresses STAT3 constitutively active mutant

    Depositor

    Kevin Park

    Article

    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

    Insert

    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    changed Alanine 662 to Cysteine and Asparagine 66…

    Promoter

    CMV enhancer and promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRL_CT_GI_del1

    Plasmid

    #98744

    Purpose

    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

    Inserts

    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    SV40-NLS, VP64-AD, and synTALE-DBD

    Expression

    Yeast

    Mutation

    mutated nucleotide 576 C to A, deleted amino acid…

    Promoter

    FBA1 and TDH3

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO_HA.V5.FLAG_NGFR

    Plasmid

    #158250

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.V5.FLAG

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CCR6-DuET

    Plasmid

    #213202

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR6 (CCR6 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH

    Plasmid

    #105981

    Purpose

    This AAV plasmid constitutively expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

    Depositor

    Marcel Bruchez

    Article

    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

    Insert

    hSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH
    

    Use

    AAV

    Tags

    FAPdL5-POST, T2A-dTomato, and myc

    Promoter

    human synapsin 1

    Available Since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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