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pHH0103_NPHP1-1/1

Plasmid

#91358

Purpose

Protein expression and purification of human SH3 domain construct NPHP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NPHP1-1/1 (NPHP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PACSIN1-1/1

Plasmid

#91372

Purpose

Protein expression and purification of human SH3 domain construct PACSIN1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN1-1/1 (PACSIN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D21-1/3

Plasmid

#91296

Purpose

Protein expression and purification of human SH3 domain construct SH3D21-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D21-1/3 (SH3D21 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO15A-2/2

Plasmid

#91291

Purpose

Protein expression and purification of human SH3 domain construct MYO15A-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO15A-2/2 (MYO15A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1 F74G -FLAG

Plasmid

#189728

Purpose

Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e KRAB

Plasmid

#188506

Purpose

Expresses FLAG tagged PlmdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-KRAB-NLS

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEYFP-Pds1Δdestruction box(383)

Plasmid

#39848

Depositor

Jonathon Pines

Article

Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

Insert

Pds1 (PTTG1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

Δdestruction box = ΔR61-N68

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB1-C-18

Plasmid

#55415

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB1 (MAPRE1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-ODC1

Plasmid

#133256

Purpose

Express FLAG-tagged ODC1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

ODC1 (ODC1 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pG4-E6s-luc

Plasmid

#46324

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

5xGal4

Use

Luciferase

Tags

E boxes and luciferase

Expression

Mammalian

Mutation

five copies of a multimerized GAL4 (5×GAL4) site …

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-FOP

Plasmid

#136796

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN E50K

Plasmid

#175765

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN E50K

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Mutation

E50K

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJOE5751.1

Plasmid

#135083

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-His tag-eGFP

Expression

Bacterial

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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SLC9A3R1_PDZ_2

Plasmid

#103933

Purpose

Protein expression and purification of SLC9A3R1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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HsSPHK2

Plasmid

#118092

Purpose

Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

Depositor

Kevin Lynch

Article

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)

Tags

FLAG

Expression

Yeast

Promoter

GAL1, 10

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-128

Plasmid

#26308

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-128 (MIR128-1 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pHH0103_PPP1R13L-1/1

Plasmid

#91509

Purpose

Protein expression and purification of human SH3 domain construct PPP1R13L-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PPP1R13L-1/1 (PPP1R13L Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCC20

Plasmid

#198783

Purpose

Drives specific expression in the ASK neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psra-9-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP RanSen

Plasmid

#184705

Purpose

Lentiviral transfer plasmid for expression of GFP RanSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP RanSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pECFP-N2-COL1A1

Plasmid

#66603

Purpose

Expresses procollagen 1 alpha 1 as a CFP fusion

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

Insert

COL1A1 (COL1A1 Human)

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only