We narrowed to 20,704 results for: HA

1. RT-4

   Plasmid

   #220443

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (PUF3 Budding Yeast)
   

   Tags

   3xHA

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMS110

   Plasmid

   #215674

   Purpose

   Split hygromycinR landing pad insertion plasmid for ChrIII

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   3' ∆HYGR is promoterless and encodes aa60-341

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA4/TO-Cdc45HA

   Plasmid

   #211427

   Purpose

   tet-inducible mammalian expression of human Cdc45 C-terminally fused to HA-Tag

   Depositor

   Helmut Pospiech

   Article

   Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

   Insert

   Cdc45 (CDC45 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX

   Plasmid

   #209712

   Purpose

   Mammailan expression of WDR5, SH2, and HRas separated by P2A.

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   WDR5-P2A-SH2-P2A-Ras-CAAX
   

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-NLS-MBP-rATOM(WDR5)

   Plasmid

   #209710

   Purpose

   Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   NLS-MBP-rATOM(WDR5)
   

   Tags

   NLS-MBP

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRV∆L-5tTA

   Plasmid

   #182966

   Purpose

   The 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   tTA
   

   Use

   Rabies

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 6xHis(TEV)-Drp1(K569A,K571A)-StrepII

   Plasmid

   #174426

   Purpose

   Expresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   K569A, K571A

   Promoter

   T7

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2+-wbp2nlY282F

   Plasmid

   #172468

   Purpose

   expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nlY282F
   

   Use

   Xenopus expression

   Tags

   none

   Mutation

   changed Tyrosine at aa282 to Phenylalanine

   Promoter

   SP6

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 6xHis(TEV)-Drp1(K557A,K560A)-StrepII

   Plasmid

   #174425

   Purpose

   Expresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   K557A, K560A

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+-wbp2nlF127P

    Plasmid

    #174507

    Purpose

    expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlF127P
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    changed Phenylalanine at aa127 to Proline

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+-wbp2nlY55F

    Plasmid

    #172466

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlY55F
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    changed Tyrosine at aa55 to Phenylalanine

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+-wbp2nl

    Plasmid

    #172463

    Purpose

    expresses wild-type protein when injected into embryos

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nl (wbp2nl.L Frog)
    

    Use

    Xenopus expression

    Tags

    none

    Mutation

    none

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBlastR-5xQUAS-EBFP2-GMRWhite

    Plasmid

    #165921

    Purpose

    Blasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBlastR-5xQUAS-sfGFP-MiniWhite

    Plasmid

    #165922

    Purpose

    Blasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBlastR-5xQUAS-EBFP2-MiniWhite

    Plasmid

    #165924

    Purpose

    Blasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable QUAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBlastR-5xUAS-EBFP2-MiniWhite

    Plasmid

    #165912

    Purpose

    Blasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. P[acman]-Alpha1

    Plasmid

    #165858

    Purpose

    Alpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P[acman]-Omega1

    Plasmid

    #165860

    Purpose

    Omega1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pR6Kg-Alpha2

    Plasmid

    #165863

    Purpose

    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBlastR-5xUAS-mCherry-GMRWhite

    Plasmid

    #165908

    Purpose

    Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge