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We narrowed to 15,064 results for: pET

Showing: 201 - 250 of 15064 results

  5. pET_BGL2

    Plasmid
    #101911
    Purpose
    Expresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.
    Depositor
    Steve Shih
    Article
    Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944
    Insert
    β-glucosidase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7+LacO
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    101911-plasmid-map-sequence-id-197171

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  8. pET28a_LolT_C41S

    Plasmid
    #211789
    Purpose
    Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S
    Depositor
    Yang Hai
    Article
    Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:
    Insert
    lolt mutant
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    211789-plasmid-map-sequence-id-420926

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  10. pet15b_ack

    Plasmid
    #220229
    Purpose
    Bacterial expression of AcK in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    Acetate kinase (ackA )
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220229-plasmid-map-sequence-id-436823

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  12. pETM33_Nsp3d_Ub2_PLPro

    Plasmid
    #156472
    Purpose
    Bacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp3d_Ub2_PLPro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156472-plasmid-map-sequence-id-307744

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  13. pET22b_EcSSB_2

    Plasmid
    #78204
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-
    Insert
    ssb (W88C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    W88C (please see depositor comment below)
    Promoter
    T7
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78204-plasmid-map-sequence-id-151737

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  16. pET30b_T7Sav

    Plasmid
    #138588
    Purpose
    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10
    Insert
    T7-tagged streptavidin variant with native C-terminus
    Use
    Synthetic Biology
    Tags
    T7-tag
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138588-plasmid-map-sequence-id-274485

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  18. pET22B_PcrA_2Bchimera

    Plasmid
    #102998
    Purpose
    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
    Depositor
    Martin Webb
    Insert
    PcrA_2Bchimera
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Bacilllus stearothermophilus PcrA in which the 2B…
    Promoter
    T7
    Available Since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102998-plasmid-map-sequence-id-199442

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  19. pET14b_tev_PDZ1

    Plasmid
    #59739
    Purpose
    expresses PDZ1 domain of human ZO-1 in bacteria
    Depositor
    Alan Fanning
    Insert
    zonula occludens-1 (TJP1 Human)
    Use
    Tags
    HIS tag and thrombin clvg
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pETM33_Nsp1

    Plasmid
    #156465
    Purpose
    Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156465-plasmid-map-sequence-id-307729

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  25. pETM33_N_CTD

    Plasmid
    #156484
    Purpose
    Bacterial expression of Sars-CoV2 N_CTD protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    N_CTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156484-plasmid-map-sequence-id-307735

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  27. pETM33_N_NTD

    Plasmid
    #156483
    Purpose
    Bacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    N_NTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156483-plasmid-map-sequence-id-307745

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  34. pet15_rbsk

    Plasmid
    #220227
    Purpose
    Bacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    ribokinase (rbsK E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220227-plasmid-map-sequence-id-436827

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  37. pET15bLCFPT7

    Plasmid
    #53545
    Purpose
    Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant
    Depositor
    Lingchong You
    Article
    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10
    Insert
    T7 RNAP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    53545-plasmid-map-sequence-id-83442

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  39. pET:GACT:GFP

    Plasmid
    #60734
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60734-plasmid-map-sequence-id-97720

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  40. pET28b_NOSER

    Plasmid
    #124244
    Purpose
    Bacterial 6xHIS tagged protein expression without the addition of any potential phosphorylation sites to the tagged protein.
    Depositor
    Attila Fehér
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124244-plasmid-map-sequence-id-240376

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  45. pETM33_Nsp7

    Plasmid
    #156476
    Purpose
    Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156476-plasmid-map-sequence-id-307742

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  48. pETM33_Nsp8

    Plasmid
    #156477
    Purpose
    Bacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156477-plasmid-map-sequence-id-307732

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Showing: 201 - 250 of 15064 results