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We narrowed to 2,347 results for: puc19

Showing: 201 - 250 of 2347 results

  2. pUC19-TSV (linearized in VP2)

    Plasmid
    #70032
    Purpose
    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region
    Depositor
    Mariet Feltkamp
    Article
    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.
    Insert
    TSV full genome, linearized in VP2 gene
    Use
    Cloning vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    70032-plasmid-map-sequence-id-129213

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  3. pUC19-link-PAGFP-FLAG:Kan

    Plasmid
    #211834
    Purpose
    Plasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.
    Depositor
    Peng Chen
    Insert
    PAGFP-FLAG-Kan
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Available Since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211834-plasmid-map-sequence-id-418061

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  5. pUC19-Top-Toehold-mCherry

    Plasmid
    #87318
    Purpose
    Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87318-plasmid-map-sequence-id-174668

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  9. Mishler2014-No-aptamer-controlluciferase-pUC19

    Plasmid
    #63856
    Purpose
    This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.
    Depositor
    Howard Salis
    Article
    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv
    Insert
    No-aptamer control mRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tac
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pUC19 KGA HA5'-mKO2-3'

    Plasmid
    #110406
    Purpose
    Homology donor for glutaminase KGA isoform knock-in, mKO2
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110406-plasmid-map-sequence-id-218847

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  28. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  42. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid
    #133793
    Purpose
    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    CjeCas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    V2-TGAA linker sgRNA architecture from Kim et al.…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133793-plasmid-map-sequence-id-268077

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  46. pUC19-SARS-CoV-2-N-gene-truncated

    Plasmid
    #240635
    Purpose
    pUC19-SARS-CoV-2-N-gene-truncated plasmid is a plasmid that contains the SARS-CoV-2 N-gene and can be used as a positive control in SARS-CoV-2 research or diagnostics assays targeting the N gene.
    Depositor
    Kevin Yehl
    Insert
    SARS-CoV-2-N-gene-truncated (N )
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    240635-plasmid-map-sequence-id-486094

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Showing: 201 - 250 of 2347 results