-
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc1/bC
Plasmid#74049Purposeretroviral expression plasmid for human NFATc1/bCDepositorInserthuman NFATc1, isoform beta-C (NFATC1 Human)
UseRetroviralTagsno tagExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-1xL
Plasmid#186907PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHept
Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
LLP787_SnoopCatcher-EZH2-FL-CO
Plasmid#211780PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-DNMT3A
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP780_SpyCatcher-KRAB-CO
Plasmid#211773PurposeSpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertSpyCatcher-KRAB
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natCX
Plasmid#73893Purposefission yeast drug selection markerDepositorInsertnatCX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher EQ
Plasmid#35045DepositorInsertSpyCatcher EQ
UseTagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…PromoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-AtC60αβC20NtL
Plasmid#160876PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: T7, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR4-huNFATc1nuc
Plasmid#23988DepositorInsertNFATc1 (NFATC1 Human)
UseCloning vectorTagsEE tagExpressionMutationS172A S175A S176A S178A S179A S181A S184A S187A S…PromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBS mNFATc3-EE
Plasmid#17868DepositorInsertNFATc3 (Nfatc3 Mouse)
UseTagsEEExpressionBacterialMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLAtC60αβC20
Plasmid#160873PurposeExpression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coliDepositorInsertrbcL, cpn60α, cpn60β, cpn20
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 V5 mDispatched WT 2x HA
Plasmid#126410PurposeExpression of V5 Dispatched WT HA in mammalian cell lines.DepositorInsertm-Dispatched1 (Disp1 Mouse)
UseTagsHAExpressionMammalianMutationV5 iserted between A106 and A107PromoterAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-GFP
Plasmid#186900PurposeCell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10DepositorInsertTfR-sfGFP-SpyCatcher003-sfCherry1-10
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Patched1-N-18
Plasmid#54216PurposeLocalization: Cilium, Excitation: 487, Emission: 509DepositorInsertPatched1
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAtCas9-D10A_2
Plasmid#91160PurposeCas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt IIDepositorInsertAtCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR4 hNFATc1-EE
Plasmid#17857DepositorInsertNFATc1 (NFATC1 Human)
UseTagsEEExpressionBacterialMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1 mNFATc2
Plasmid#17858DepositorInsertNFATc2 (Nfatc2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only