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We narrowed to 390 results for: PEF1

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LLP250 pEF1a-TALE-SunTag

Plasmid

#100939

Purpose

Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE insertion site, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1α-owl-monkey-retroCHMP3-HA

Plasmid

#154249

Purpose

expresses owl monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a_ TET1 CXXC only

Plasmid

#124084

Purpose

Expression of CXXC domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - CXXC domain (TET1 Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

EF1 alpha

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1-puro/hpb1(short)

Plasmid

#59973

Purpose

integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression

Depositor

Timothy Springer

Insert

human integrin b1 (short) (ITGB1 Human)

Use

Tags

Base coiled-coil and His-tag

Expression

Mammalian

Mutation

Promoter

EF1a promoter

Available since

Sept. 24, 2014

Availability

Academic Institutions and Nonprofits only
LLP251 pEF1a-UNC5C-4-SunTag

Plasmid

#100940

Purpose

TALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE target sequence for UNC5C, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP245 pEF1a-TALE-DNMT3a-Wt

Plasmid

#100937

Purpose

Empty TALE vector driven by EF1a promoter, no Puro, contains DNMT3a catalytic domain

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE insertion site, DNMT3a catalytic domain (DNMT3A Synthetic, Human)

Use

TALEN

Tags

3xHA and 3xTy1

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP338 pEF1a-DACH1-1-SunTag

Plasmid

#100942

Purpose

TALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE target sequence for DACH1, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-CHMP3(147)-HA

Plasmid

#154177

Purpose

expresses mouse CHMP3(147) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 147

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

Plasmid

#69583

Purpose

Expresses p53 tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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LLP469 pEF1a-mCherry-EMPTY-gRNA

Plasmid

#100958

Purpose

mCherry driven by EF1a, empty gRNA scaffold driven by U6

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

gRNA empty backbone with RFP

Use

CRISPR

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

Plasmid

#100941

Purpose

Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)

Use

Tags

1xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223252

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEf1a OsTIR ires NEO pA T2BH

Plasmid

#127910

Purpose

Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

OsTIR

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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LLP453 pEf1a-NLS-aGCN4-mCherry

Plasmid

#100945

Purpose

mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, mCherry

Use

Tags

3xTy1

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-Ronin-FLAG-IRES-Neo

Plasmid

#28020

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-full-length-CHMP3-HA

Plasmid

#154176

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

Plasmid

#69587

Purpose

Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344P (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

p53 L344P mutation that prevents dimerization and…

Promoter

EF1alpha

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

Plasmid

#69582

Purpose

Expresses p53 tagged with mKate2 and split N-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - N term

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1α-squirrel-monkey-CHMP3(155)-HA

Plasmid

#154173

Purpose

expresses squirrel monkey CHMP3(155) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3(155)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 155

Promoter

EF1-alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-RoninDHSA-FLAG-IRES-Neo

Plasmid

#28021

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

DHSA mutation (Y246A) of the previously defined H…

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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