We narrowed to 460 results for: PUR ALPHA-1

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pLVX MLKL(1-180)-2A-mCherry-puro

Plasmid

#231976

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBabe puro Myr HA PIK3CB

Plasmid

#12527

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CB (PIK3CB Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Myristoylation. Constitutively active allele.

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PIK3C2A KD5

Plasmid

#162006

Purpose

PIK3C2alpha shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Canis Lupus)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti puro HA-TUBA1B

Plasmid

#227587

Purpose

Lentiviral plasmid expressing human TUBA1B protein

Depositor

Melina Fan, Addgene Research Program

Insert

TUBA1B (TUBA1B Human)

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro

Plasmid

#116876

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TRAV10 - P2A - TRBV25 (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a mNeonGreen-3K-B1-10-IRES-mKate2-2A-PuroR

Plasmid

#215377

Purpose

Lentiviral expression of a cytosolic-localized split mNeonGreen variant that enables efficient complementation when the missing beta strand is present. mKate2 is present as a transduction control.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

mNeonGreen-3K-1-10

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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OmEF1aCas9P2ApuroSB

Plasmid

#165484

Purpose

Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

Depositor

Dietmar Kueltz

Article

Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

Insert

Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)

Use

CRISPR; Transposon

Promoter

Oreochromis mossambicus EF1 alpha promoter (OmEF1…

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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N111 nLV EF-1a-PGK-Puro Gal4-HP1a FL

Plasmid

#43968

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Gal4/DBD

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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N112 nLV EF-1a-PGK-Puro Gal4-HP1a CS

Plasmid

#43969

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Gal4/DBD

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P531A-pBabe-puro

Plasmid

#26091

Depositor

William Kaelin

Article

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA with point mutation P531A

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin

Plasmid

#133026

Purpose

Expresses EGFP-tagged tail anchor sequence of Bik

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Bcl-2-interacting killer (BIK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-110

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro

Plasmid

#44195

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Frb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

Plasmid

#133028

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Golgin84 (GOLGA5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-673

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

Plasmid

#133025

Purpose

Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Monoamine oxidase type A (MAOA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-488

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A;P531A;bHLH*-pBabe-Puro

Plasmid

#22135

Depositor

William Kaelin

Article

Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids from wt: Proline 405 t…

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only

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Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124287

Purpose

sgRNA against human Col7A1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124286

Purpose

sgRNA against human Col7A1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-miRFP670-eDHFR(69K6)

Plasmid

#178852

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-mNG-eDHFR(69K6)

Plasmid

#178853

Purpose

Mammalian expression of mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-surfaceHA-IRES-Puro

Plasmid

#207321

Purpose

Lentiviral expression of synthetic HA surface receptor in Mammalian cells

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HAtag-Myctag-PDGFRB-TM-IRES-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha_short (EFS)

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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