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Plasmid#222479PurposeCo-expression of two genes in yeast with FLAG and MYC tags and THR1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only
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pESC-ADE
Plasmid#222480PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ADE2 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ARG
Plasmid#222481PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ARG1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESUMO-hRORg1
Plasmid#170330PurposeExpresses RORg isoform 1 with N-terminal HisSUMO tag in bacteriaDepositorInsertRORG isoform 1 (RORC Human)
UseTagsHisSUMOExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-Hypa
Plasmid#218165PurposeThis plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in StreptomycesDepositorInsertsescbe3-NG-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKAd5f11pES-PmeI
Plasmid#122698PurposeTo generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.DepositorTypeEmpty backboneUseAdenoviralTagsExpressionMutationPromoterAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS-PesaRlux
Plasmid#47640Purposewt esaR promoter in pCS26DepositorInsertPesaR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPesaRAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaRlux
Plasmid#47802PurposePesaR promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-luxCDABE-term
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPesaRAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBPhsFlp2::PEST
Plasmid#63171PurposeDrosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock PromoterDepositorInserthsFlp2::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+pEstrInd
Plasmid#170880PurposePhytobrick - Estradiol Inducible PromoterDepositorInsertpEstradiol_Inducible
UseTagsExpressionPlantMutationc.837C>T and c.1353G>A to remove BsaI and E…PromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC/mCitrine
Plasmid#117996PurposeExpression of mCitrine controlled by the Gal1 promoterDepositorInsertmCitrine
UseYeast/bacteria binary vectorTagsExpressionYeastMutationPromoterGal1Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1
Plasmid#218164PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESR1-donor
Plasmid#112337PurposeCRISPR donor plasmid to tag human transcription factor ESR1 with GFPDepositorInsertESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESR1.1.0-gDNA
Plasmid#112433PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1DepositorInsertESR1 (ESR1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
peSpCas9-J23109
Plasmid#113152PurposePlasmid constitutively expressing eSpCas9 proteinDepositorInserteSpCas9
UseTagsExpressionBacterialMutationK810A & K1003A & R1060APromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
peSpdCas9-J23109
Plasmid#113153PurposePlasmid constitutively expressing eSpdCas9 proteinDepositorInserteSpdCas9
UseTagsExpressionBacterialMutationD10A & K810A & H840A & K1003A & R…PromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pESUMO-hRORg2
Plasmid#170329PurposeExpresses RORg isoform 2 with N-terminal HisSUMO tag in bacteriaDepositorInsertRORG isoform 2 (RORC Human)
UseTagsHisSUMOExpressionBacterialMutationcodon optimizedPromoterT7Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC/CURT_fluoB
Plasmid#117997PurposeExpression of CURT_fluoB controlled by the Gal1 promoterDepositorInsertCURT_fluoB
UseYeast/bacteria binary vectorTagsExpressionYeastMutationPromoterGal1Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEsg1-6K-Luc
Plasmid#15394DepositorInsertUpstream region of mouse Esg1 gene (Dppa5a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-PES-H1
Plasmid#218704PurposeEncodes codon optimized PES-H1 enzyme for bacterial expressionDepositorInsertPES-H1
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only