We narrowed to 8,022 results for: ARA-2

1. pHAGE-CMV-Rheb-IRES-eGFP-W

   Plasmid

   #32519

   Depositor

   Mustafa Sahin

   Article

   Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

   Insert

   Rheb (Rheb Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pCI pHluorin Syt2

   Plasmid

   #184503

   Purpose

   Mammalian expression of Synaptotagmin 2 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 2 (Syt2 Mouse)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KA2628_pGL4-UBCmin-luc2/Tbx3-B'

   Plasmid

   #124201

   Purpose

   Luciferase reporter driven by the Tbx3 enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Tbx3 enhancer
   

   Use

   Luciferase

   Promoter

   UBCmin

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZ003 (LshC2c2 locus)

   Plasmid

   #70168

   Purpose

   Expresses LshC2c2 full locus

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   LshC2c2 full locus
   

   Expression

   Bacterial

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZ001 (AacC2c1 locus)

   Plasmid

   #70166

   Purpose

   Expresses AacC2c1 full locus

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   AacC2c1 locus
   

   Expression

   Bacterial

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Dapp1-PH pET11a

   Plasmid

   #71376

   Purpose

   Phosphoinositide Binding

   Depositor

   Mark Lemmon

   Article

   Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

   Insert

   Dapp1-PH (DAPP1 Human)
   

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 148-273

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pSCL.028

   Plasmid

   #184965

   Purpose

   Test effect of extending -Eco1 a1/a2 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(extended), a1/a2 length: 27
   

   Expression

   Yeast

   Mutation

   human codon optimized RT, a1/a2 length extended t…

   Promoter

   Gal7

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KA1158_pPBCAG-MerCreMer-IZ

   Plasmid

   #124184

   Purpose

   piggyBac-based expression of the tamoxifen-inducible Cre

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   MerCreMer-IRES-Zeo
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmPB3-VSV

   Plasmid

   #68039

   Purpose

   Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Plexin-B3 (Plxnb3 Mouse)
   

   Tags

   VSV

   Expression

   Mammalian

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KA1875_pGL4-UBCmin-luc2/Sox2_SRR2

    Plasmid

    #124198

    Purpose

    Luciferase reporter driven by the Sox2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Sox2 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSC201

    Plasmid

    #129387

    Purpose

    A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.

    Depositor

    Silvia Cardona

    Article

    Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do

    Insert

    oriR6K and oriT
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    N/A

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KA1868_pGL4-UBCmin-luc2/Utf1-A'

    Plasmid

    #124194

    Purpose

    Luciferase reporter driven by the Utf1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Utf1 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KG#59

    Plasmid

    #110929

    Purpose

    Expresses proteins pan-neuronally in C. elegans (entire nervous system)

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    unc-54 3' control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KA1870_pGL4-UBCmin-luc2/Esrrb-B'

    Plasmid

    #124196

    Purpose

    Luciferase reporter driven by the Esrrb enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Esrrb enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KA1869_pGL4-UBCmin-luc2/Fgf4-A'

    Plasmid

    #124195

    Purpose

    Luciferase reporter driven by the Fgf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Fgf4 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLS.546

    Plasmid

    #184963

    Purpose

    Test effect of a1/a2 length on editing priB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
    

    Expression

    Bacterial

    Mutation

    priB donor G1069A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KG#470

    Plasmid

    #110930

    Purpose

    Expresses proteins pan-neuronally in C. elegans (entire nervous system)

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    unc-54 3' control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSLS.540

    Plasmid

    #184959

    Purpose

    Test effect of a1/a2 length on editing fabH using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
    

    Expression

    Bacterial

    Mutation

    fabH donor G954A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KA1874_pGL4-UBCmin-luc2/Sox2_SRR1

    Plasmid

    #124197

    Purpose

    Luciferase reporter driven by the Sox2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Sox2 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KA2627_pGL4-UBCmin-luc2/Jam2-A'

    Plasmid

    #124200

    Purpose

    Luciferase reporter driven by the Jam2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Jam2 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. KA1867_pGL4-UBCmin-luc2/Pou5f1_CR2

    Plasmid

    #124193

    Purpose

    Luciferase reporter driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Pou5f1 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. KA1866_pGL4-UBCmin-luc2/Pou5f1_CR3

    Plasmid

    #124192

    Purpose

    Luciferase reporter driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Pou5f1 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. KA1865_pGL4-UBCmin-luc2/Pou5f1_CR4

    Plasmid

    #124191

    Purpose

    Luciferase reporter driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Pou5f1 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. KA1403_pGL4-UBCmin-luc2/Nanog-B'

    Plasmid

    #124190

    Purpose

    Luciferase reporter driven by the Nanog enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Nanog enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KA1402_pGL4-UBCmin-luc2/Nanog-A'

    Plasmid

    #124189

    Purpose

    Luciferase reporter driven by the Nanog enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Nanog enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPKm-145

    Plasmid

    #90505

    Purpose

    Empty plasmid, pSIN-EF1-alpha-IRES-puro

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CRY2(delta2-6)-mCherry

    Plasmid

    #104182

    Purpose

    Expresses fusion of CRY2(delta aa 2-6) with mCherry

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2(delta2-6)-mCherry
    

    Expression

    Mammalian

    Mutation

    CRY2PHR with deletion of aa 2-6

    Promoter

    CMV

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Osa-pCAMBIA-1301-STTM408-3p

    Plasmid

    #84217

    Purpose

    Disturb the expression of miR408-3p in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR408-3p
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSCL.031

    Plasmid

    #184968

    Purpose

    Test effect of extending -Eco2 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco2: RT and ncRNA(extended), a1/a2 length: 29
    

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSCL.72

    Plasmid

    #184984

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker1-Eco1RT
    

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSCL.95

    Plasmid

    #184986

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker2-Eco1RT
    

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSLS.544

    Plasmid

    #184961

    Purpose

    Test effect of a1/a2 length on editing murF using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    

    Expression

    Bacterial

    Mutation

    murF donor G1359A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSCL.94

    Plasmid

    #184985

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker2-Cas9
    

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSCL.71

    Plasmid

    #184983

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker1-Cas9
    

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. L1-AA027

    Plasmid

    #185752

    Purpose

    Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Insert

    HygR2
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

    Plasmid

    #124181

    Purpose

    piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Promoter

    UBCmin

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

    Plasmid

    #124180

    Purpose

    piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    EGFP-IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Promoter

    UBCmin

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-YJR129c

    Plasmid

    #85118

    Purpose

    bacterial expression of YJR129c

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFM3 (EFM3 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Osa-pCAMBIA-1301-STTM408-5p

    Plasmid

    #84218

    Purpose

    Disturb the expression of miR408-5p in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR408-5p
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Osa-pCAMBIA-1301-STTM530-3p

    Plasmid

    #84226

    Purpose

    Disturb the expression of miR530-3p in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR530-3p
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. CRY2olig-mCherry

    Plasmid

    #60032

    Purpose

    Expresses a fusion of CRY2PHR E490G with mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2PHR E490G (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Fragment1 with TLS2

    Plasmid

    #196983

    Purpose

    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Intermediate fragment for assembly of gRNA-TLS2 construct
    

    Use

    Gateway-compatible entry vector

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. B2094_CRY2olig-miRFP

    Plasmid

    #215114

    Purpose

    Expresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    CRY2olig-miRFP (CRY2 Mustard Weed)
    

    Tags

    miRFP

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDBTrp-LexABD-CRY2FL

    Plasmid

    #78210

    Purpose

    Use with Addgene 40615 for blue light transcription control in yeast

    Depositor

    Chandra Tucker

    Article

    Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

    Insert

    LexABD-CRY2 (CRY2 Mustard Weed)
    

    Tags

    lexA binding domain

    Expression

    Yeast

    Mutation

    contains endogenous CRY2 NLS

    Promoter

    ADH1

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. p5E-pth2

    Plasmid

    #191428

    Purpose

    Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)

    Depositor

    Timothy Erickson

    Article

    Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi

    Insert

    pth2 regulatory region (pth2 Zebrafish)
    

    Use

    Tol2 gateway 5' entry vector

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pVH016

    Plasmid

    #80400

    Purpose

    No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. attB-P7-attP-YFP

    Plasmid

    #195571

    Purpose

    A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.

    Depositor

    Richard Murray

    Article

    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

    Insert

    Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
    

    Use

    Synthetic Biology

    Promoter

    P7

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid

    #160138

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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