Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)
Plasmid
#217803
Purpose
Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
Depositor
Insert
mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
Use
CRISPR; In vitro transcription; t7 promoter
Tags
BPNLS
Expression
Mammalian
Mutation
nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
Promoter
CMV and T7
Available Since
Sept. 6, 2024
Availability
Academic Institutions and Nonprofits only