We narrowed to 4,733 results for: GCA

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pCfB3052(gRNA X-4, XI-3, XII-5)

Plasmid

#73294

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pUDP013

Plasmid

#103873

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (mPCSK9)

Plasmid

#170122

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Pcsk9 Mouse)

Use

AAV

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hsf-1-sgRNA-A

Plasmid

#177786

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hsf-1 (hsf-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL1-pegRNA-attR5

Plasmid

#213053

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA2_ASCL1

Plasmid

#64130

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL2 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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eGFP-SUN1

Plasmid

#213515

Purpose

Expresses human eGFP-SUN1 in mammalian cells

Depositor

Karim Mekhail

Article

Shokrollahi et al Nat Struct Mol Biol. 2024 Sep;31(9):1319

Insert

SUN1 (SUN1 Human)

Expression

Mammalian

Mutation

SUN1 has a silent mutation on its gene (nucleotid…

Promoter

CMV

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pX-EGFP-g1

Plasmid

#107273

Purpose

eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

GFP sgRNA-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA1.1

Plasmid

#64116

Purpose

Vector for expression of St1 sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-hHSF2 shRNA

Plasmid

#118346

Purpose

This plasmid expresses shRNA against hHSF2.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF2 shRNA (HSF2 Human)

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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shYES1 # 1

Plasmid

#42546

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pCoHygro L16_shRNA of spliced Copia

Plasmid

#71389

Purpose

knock down expression of spliced Copia in Drosophila melanogaster

Depositor

Timothy Nilsen

Insert

Copia

Expression

Insect

Promoter

U6

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pmIL-6 mut AP-1+C/EBP

Plasmid

#61292

Purpose

drives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sites

Depositor

Gail Bishop

Article

Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Insert

IL-6 promoter (Il6 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Mutated both AP-1 binding site from TGAGTCT to TG…

Promoter

IL-6 promoter with mutant AP-1 and C/EBP binding …

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only
pCR8-attR4-pegRNA-attR3

Plasmid

#213056

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attL4

Plasmid

#213055

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP-Ofd1-Myc-A80T

Plasmid

#24579

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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PRKDC gRNA (BRDN0001149438)

Plasmid

#77863

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKDC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKDC (PRKDC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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