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Plasmid#65517PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only
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GFP-muSOX
Plasmid#131701Purposeexpresses muSOX with GFP N-terminally fusedDepositorInsertGFP-muSOX (GAMMAHV.ORF37 MHV68)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCO604
Plasmid#139737Purposeindividual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnH3HDepositorInsertH3H
UseLuciferase; Moclo level 0 componentTagsExpressionMutationPromoternoneAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
N-GFP-Dcp2
Plasmid#163585Purposeexpress N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterDCP2 promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-miniTurboID-EGFP
Plasmid#163847PurposeIn vitro transcription of miniTurboID-EGFP. Parton lab clone JRTDepositorInsertminiTurbo
UseIn vitro transcription of rnaTagsEGFPExpressionMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP42
Plasmid#65518PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionYeastMutationPromoterpPMA1Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP35
Plasmid#65513PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP32
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastMutationPromoterpPMA1Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSynapsin SV-tag
Plasmid#163685Purposeexpress SV-Tag construct in a Cre-independent mannerDepositorInsertsynatophysin 9X HA tag T2A tdtomato
UseTags9X HAExpressionMammalianMutationPromotersynapsinAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-3H-GFP
Plasmid#205725PurposeExpression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundleDepositorInsertpOTC-3H-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-2 SNAP-Syk tSH2
Plasmid#113028Purposefor purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.DepositorInsertSNAP-Syk tSH2
UseTagsSNAPExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCO486
Plasmid#139729PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, NpgaDepositorInsertpMOD_A_35S Short_TMV 5':AsNpga:tAtug7
UseLuciferaseTagsExpressionPlantMutationPromoter35SAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Talin Head
Plasmid#186140PurposeFor expression of recombinant Talin head domain in bacteriaDepositorInsertTLN1 (TLN1 Human)
UseTagsHis6 (tev cleavage)ExpressionBacterialMutationPromoterLacAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptKS(-)-FRB
Plasmid#104475PurposePlasmid carrying FRB with C-terminal HA tag.DepositorInsertFRB (MTOR Human)
UsePhagemidTagsHA tagExpressionMutationincludes only the FRB domainPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::3xHalo7
Plasmid#87649PurposeFor creating drosophila transgenics expressing UAS-Syt::3xHalo7DepositorInsertSyt::3xHalo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYN1-fDIO-rtTA
Plasmid#166598PurposeEncodes Flp-dependent rtTADepositorInsertrtTA
UseAAVTagsExpressionMutationPromoterhuman synapsin 1Available sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-deltaR8.2(A14C,E45C,A92E)
Plasmid#79048Purposemammalian expression, lentiviral packagingDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Gag-delta-p6-mEGFP
Plasmid#116890Purposeexpresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cellsDepositorInsertsynthetic Gag missing the p6 domain
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVermilion-C1
Plasmid#105756PurposeEmpty vector for creating N terminal mVermilion fusionsDepositorTypeEmpty backboneUseTagsmVermilionExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
P366
Plasmid#139722PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, NpgaDepositorInsertpMOD_A''_pSlRBCS2:AsNpga:tAtuG7
UseLuciferaseTagsExpressionPlantMutationPromoterSlRBCS2Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only