We narrowed to 2,567 results for: gem

1. p006-Banana-Late

   Plasmid

   #112251

   Purpose

   ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   ATF1 under osmY promoter
   

   Expression

   Bacterial

   Promoter

   osmY

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p005-RFP-strong

   Plasmid

   #108317

   Purpose

   Synthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   RFP under controlf of strong synthetic constitutive promoter pFAB4005
   

   Expression

   Bacterial

   Promoter

   pFAB4005

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-puro-shFAM134B

   Plasmid

   #109013

   Purpose

   short-hairpin knockdown of targeted gene

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   shFAM134B (RETREG1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CaMKIIa-ChETA-EYFP

   Plasmid

   #26967

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T

   Promoter

   CaMKIIa

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. rLAMP1-mCherry-EGFP-Tet-On

   Plasmid

   #109015

   Purpose

   Tet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   rLAMP1
   

   Use

   Lentiviral

   Tags

   mcherry-eGFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBLego

   Plasmid

   #219967

   Purpose

   The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.

   Depositor

   Markus Seeger

   Article

   Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10

   Type

   Empty backbone

   Tags

   SLEHHHHHH and pelB signal sequence

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

   Plasmid

   #20294

   Purpose

   Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Serine

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGCG26

   Plasmid

   #82371

   Purpose

   YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   YFP
   

   Promoter

   PJ23119

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pALM179

   Plasmid

   #82023

   Purpose

   an updated version of cLac143 (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression

   Depositor

   Brian Pfleger

   Article

   Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

   Insert

   YFP
   

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas49

    Plasmid

    #82391

    Purpose

    rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    rbcL-GFP
    

    Promoter

    PccmK2

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. p005kanRFP

    Plasmid

    #58533

    Purpose

    RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Red fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T5 between two lac operators

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Venus Cam KII alpha mutant T286D

    Plasmid

    #29429

    Depositor

    Steven Vogel

    Article

    Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

    Insert

    Venus CAM KII Alpha
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    T286D mutation in CamKIIa sequence

    Available Since

    July 5, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

    Plasmid

    #20293

    Purpose

    Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Alanine

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB3C5-proD-B0032-E0051

    Plasmid

    #107241

    Purpose

    Expresses lacZa-GFP fusion from constitutive proD promoter

    Depositor

    Joseph Davis, Robert Sauer

    Article

    Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

    Insert

    ProA-RBS(B0032)-Gemini (E0051)
    

    Promoter

    proD

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Venus Cam KII alpha mutant T286A

    Plasmid

    #29430

    Depositor

    Steven Vogel

    Article

    Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

    Insert

    Venus CAM KII Alpha
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    T286A mutation in CamKIIa sequence

    Available Since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

    Plasmid

    #20295

    Purpose

    Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Threonine

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSB3C5-proA-B0032-E0051

    Plasmid

    #107244

    Purpose

    Expresses lacZa-GFP fusion from constitutive proA promoter

    Depositor

    Joseph Davis, Robert Sauer

    Article

    Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

    Insert

    ProA-RBS(B0032)-Gemini (E0051)
    

    Promoter

    proA

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB3C5-proC-B0032-E0051

    Plasmid

    #107242

    Purpose

    Expresses lacZa-GFP fusion from constitutive proC promoter

    Depositor

    Joseph Davis, Robert Sauer

    Article

    Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

    Insert

    ProC-RBS(B0032)-Gemini (E0051)
    

    Promoter

    proC

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSB3C5-proB-B0032-E0051

    Plasmid

    #107243

    Purpose

    Expresses lacZa-GFP fusion from constitutive proB promoter

    Depositor

    Joseph Davis, Robert Sauer

    Article

    Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

    Insert

    ProB-RBS(B0032)-Gemini (E0051)
    

    Promoter

    proB

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pALM174

    Plasmid

    #82022

    Purpose

    an updated version of cLac94 (pACSA_PcptOO_YFP_PMB2__LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression

    Depositor

    Brian Pfleger

    Article

    Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

    Insert

    YFP
    

    Promoter

    Pcptoo

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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