We narrowed to 2,503 results for: gem

1. pGCG26

   Plasmid

   #82371

   Purpose

   YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PJ23119

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pALM179

   Plasmid

   #82023

   Purpose

   an updated version of cLac143 (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression

   Depositor

   Brian Pfleger

   Article

   Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

   Insert

   YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas49

   Plasmid

   #82391

   Purpose

   rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   rbcL-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PccmK2

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p005kanRFP

   Plasmid

   #58533

   Purpose

   RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   Red fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5 between two lac operators

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Venus Cam KII alpha mutant T286D

   Plasmid

   #29429

   Depositor

   Steven Vogel

   Article

   Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

   Insert

   Venus CAM KII Alpha
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   T286D mutation in CamKIIa sequence

   Promoter

   Available since

   July 5, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

   Plasmid

   #20293

   Purpose

   Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Alanine

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB3C5-proD-B0032-E0051

   Plasmid

   #107241

   Purpose

   Expresses lacZa-GFP fusion from constitutive proD promoter

   Depositor

   Robert Sauer, Joseph Davis

   Article

   Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

   Insert

   ProA-RBS(B0032)-Gemini (E0051)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   proD

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Venus Cam KII alpha mutant T286A

   Plasmid

   #29430

   Depositor

   Steven Vogel

   Article

   Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

   Insert

   Venus CAM KII Alpha
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   T286A mutation in CamKIIa sequence

   Promoter

   Available since

   June 3, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

   Plasmid

   #20295

   Purpose

   Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Threonine

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSB3C5-proA-B0032-E0051

    Plasmid

    #107244

    Purpose

    Expresses lacZa-GFP fusion from constitutive proA promoter

    Depositor

    Robert Sauer, Joseph Davis

    Article

    Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

    Insert

    ProA-RBS(B0032)-Gemini (E0051)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    proA

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSB3C5-proC-B0032-E0051

    Plasmid

    #107242

    Purpose

    Expresses lacZa-GFP fusion from constitutive proC promoter

    Depositor

    Robert Sauer, Joseph Davis

    Article

    Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

    Insert

    ProC-RBS(B0032)-Gemini (E0051)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    proC

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB3C5-proB-B0032-E0051

    Plasmid

    #107243

    Purpose

    Expresses lacZa-GFP fusion from constitutive proB promoter

    Depositor

    Robert Sauer, Joseph Davis

    Article

    Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

    Insert

    ProB-RBS(B0032)-Gemini (E0051)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    proB

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pALM174

    Plasmid

    #82022

    Purpose

    an updated version of cLac94 (pACSA_PcptOO_YFP_PMB2__LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression

    Depositor

    Brian Pfleger

    Article

    Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

    Insert

    YFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Pcptoo

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAWp9-5

    Plasmid

    #73856

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCas61

    Plasmid

    #82397

    Purpose

    IPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpK

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    LDH
    

    Use

    Tags

    Expression

    Mutation

    V39R

    Promoter

    cLac143

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAWp9-4

    Plasmid

    #73855

    Purpose

    pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Insert

    UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pEP17

    Plasmid

    #229234

    Purpose

    PutP (Sodium/proline symporter) under tet operator, TetR

    Depositor

    Yolanda Schaerli

    Article

    Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

    Insert

    putP (Sodium/proline symporter) (putP E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tet operator

    Available since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

    Plasmid

    #225438

    Purpose

    Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

    Depositor

    Diego Orzaez

    Insert

    CBS-FRT-OCS-BeYDVRepRepA-tNos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

    Plasmid

    #225430

    Purpose

    OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter

    Depositor

    Diego Orzaez

    Insert

    miniDFR-FRT-OCSterm-FRT-miniDFR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM284

    Plasmid

    #226122

    Purpose

    Expression of cdc48 E588Q

    Depositor

    Andreas Martin

    Article

    Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

    Insert

    His6-3C-Cdc48 E588Q (CDC48 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E588Q

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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