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Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-53~77-mEGFP(A206K)-TwStrep
Plasmid#208632Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 53~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~77-mEGFP(A206K)-TwStrep
Plasmid#208624Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-His8-aCat(660-865)
Plasmid#203335PurposeBacterial expression of alpha-catenin F-actin binding domainDepositorInsertCTNNA1 (CTNNA1 Human)
UseTags8xHis with precision protease cleavage siteExpressionBacterialMutationaa 660-865 onlyPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His8-aCat(660-885)
Plasmid#203334PurposeBacterial expression of alpha-catenin F-actin binding domainDepositorInsertCTNNA1 (CTNNA1 Human)
UseTags8xHis with precision protease cleavage siteExpressionBacterialMutationaa 660-885 onlyPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgβ-anticalin-His-ybbr
Plasmid#168040PurposeExpresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags6x Histag, Fgβ peptide, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Avi-Coh (E154C)-HIS
Plasmid#153447PurposeE. coli expression of Rc. CohE construct with E154C mutation.DepositorInsertRc.CohE
UseTags6xHis and Avi-tagExpressionBacterialMutationE154C mutation on CohEPromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SznF H407A H409A H448A
Plasmid#139566Purposeexpressed SznF H407A H409A H448A in E. coliDepositorInsertSznF H407A H409A H448A
UseTagshexahistidineExpressionBacterialMutationchanged glutamate 215 to alaninePromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet28a His6-HSPB7 (zebrafish) C49S
Plasmid#110071PurposeExpress C49S mutant of Zebrafish His6-HSPB7 in bacteriaDepositorInsertHis6-HSPB7 (hspb7 Zebrafish)
UseTagsHis6ExpressionBacterialMutationCysteine 49 to SerinePromoterT7Available sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FFSARG-ddFLN4-HIS-ybbr
Plasmid#101240Purposeminimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFFSARG peptide, ddFLN4 fingerprint
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 Q147A, M151A, I154A
Plasmid#74616PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationQ147A, M151A, I154APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 N68A, W69A, K77A
Plasmid#74619PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Spec(x2)-DocI
Plasmid#58713PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertSpec(x2)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(162-611)
Plasmid#39337DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B1-DK
Plasmid#117981PurposeSdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B1
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B2-DK
Plasmid#117982PurposeSdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrD_B1-DK
Plasmid#117983PurposeSdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 BD (522-645, C643A)/pET28HMT
Plasmid#111073PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 BD (522-645, C643A)PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Pet28a His6-HSPB7 (zebrafish) C117S
Plasmid#110072PurposeExpress C117S mutant of Zebrafish His6-HSPB7 in bacteriaDepositorInsertHis6-HSPB7 (hspb7 Zebrafish)
UseTagsHis6ExpressionBacterialMutationCysteine 117 to SerinePromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only