We narrowed to 11,627 results for: VARS

1. MSGV Mouse Acceptor PGK NGFR

   Plasmid

   #128482

   Purpose

   A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9

   Depositor

   David Ott

   Article

   Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

   Type

   Empty backbone

   Use

   Retroviral

   Promoter

   MSCV

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCMV-H2B-FT-Medium

   Plasmid

   #157670

   Purpose

   Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

   Depositor

   Shangqin Guo

   Article

   Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

   Insert

   Histone H2B
   

   Use

   Retroviral

   Tags

   Medium-FT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF0690 Ef1a-Kp03_attB-BFP acceptor

   Plasmid

   #193473

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCX-SpiCee-NES

   Plasmid

   #140901

   Purpose

   mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NES
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57 APEX

   Plasmid

   #40306

   Purpose

   A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

   Insert

   pea APEX (Synthetic)
   

   Use

   Unspecified

   Mutation

   K14D, W41F, E112K (relative to wild-type)

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES-Ap2a2-FKBP

   Plasmid

   #46944

   Purpose

   Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

   Depositor

   Margaret Robinson

   Article

   Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

   Insert

   Ap2a2-FKBP
   

   Tags

   Mainly mouse Ap2a2 cDNA, with a fragment of brain…

   Expression

   Mammalian

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFX-mCherry-KRasCT

   Plasmid

   #175835

   Purpose

   Express mCherry with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with plasma membrane-targeting sequence
   

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCX-NTOM-SpiCee

   Plasmid

   #140902

   Purpose

   mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   NTOM-SpiCee
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCMV-H2B-FT-Fast

   Plasmid

   #157669

   Purpose

   Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

   Depositor

   Shangqin Guo

   Article

   Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

   Insert

   Histone H2B
   

   Use

   Retroviral

   Tags

   Fast-FT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCMV-H2B-FT-Slow

    Plasmid

    #157671

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Slow-FT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. NET29A.Hs pEGFP-N2 (1181)

    Plasmid

    #62040

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET29A (TMEM120A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Splice variant

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP GLTP

    Plasmid

    #170746

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

    Insert

    GLTP (GLTP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPS0254

    Plasmid

    #115506

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCX-Lyn-SpiCee

    Plasmid

    #140837

    Purpose

    mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    Lyn-SpiCee
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCX-SpiCee-Kras

    Plasmid

    #140838

    Purpose

    mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-Kras
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1α-DIO-ReaChR-P2A-dTomato

    Plasmid

    #114368

    Purpose

    expresses a Cre-dependent red light-shifted channelrhodopsin variant

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    ReaChR-P2A-dTomato
    

    Use

    AAV

    Tags

    dTomato

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAD-SPS6-Pman

    Plasmid

    #133906

    Purpose

    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(SPS6)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pman

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

    Plasmid

    #45579

    Purpose

    Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani K227M
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-sCAG-eGFP-ACAP1 F280E

    Plasmid

    #203801

    Purpose

    AAV vector plasmid expressing human ACAP1 mutation F280E fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    ACAP1 (ACAP1 Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    F280E

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTEV5_ RpMatB_2

    Plasmid

    #78206

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

    Insert

    matB (His6 C106A S170A R286C Q457C K488A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A S170A R286C Q457C K488A

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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