We narrowed to 80,162 results for: ELL

1. pCSDest2-NmeCas9-NLS-3XHA-NLS

   Plasmid

   #87448

   Purpose

   Mammalian expression of wild type Nme Cas9

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Nme Cas9
   

   Use

   CRISPR

   Tags

   3XHA, SV40 NLS, and cMyc-like NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBeast-pAhpC-RBS- LacZ

   Plasmid

   #190060

   Purpose

   LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   lacZ
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lck-mCherry-RGS

   Plasmid

   #112935

   Purpose

   Plasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Mol Biol Cell. 2017 Nov 7;28(23):3371-33

   Insert

   p115RhoGEF-RGS domain
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. DCT.VV.Orf106

   Plasmid

   #12794

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf106
   

   Expression

   Mammalian

   Available Since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

5. CB6-GFP-TAOK1

   Plasmid

   #197108

   Purpose

   Expression of GFP-tagged TAOK1 / PSK2 in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

   Insert

   TAOK1 (TAOK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Wild Type

   Promoter

   CMV

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP3-bio

   Plasmid

   #47731

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP3
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRESneo2 mycBioID-Tollip

   Plasmid

   #178054

   Purpose

   myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Tags

   mycBioID

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-IFT54

   Plasmid

   #218726

   Purpose

   Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

   Insert

   IFT54 (TRAF3IP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mChF-Cav1s

   Plasmid

   #171464

   Purpose

   Expresses mChF-Cav1s, a FiCE of iCMM, in mammalian cells

   Depositor

   Takafumi Miyamoto

   Article

   Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

   Insert

   mCherry-FKBP12-Caveolin1(61-178 aa)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TIA1 RRM-YFP-SspBx6

    Plasmid

    #103781

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    TIA1 RRM-YFP-SspBx6
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP2-bio

    Plasmid

    #47710

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP2
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-ubiquitin-SV40-RFP

    Plasmid

    #65448

    Purpose

    Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    SV40-RFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBQ

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. VanGlow-RR

    Plasmid

    #83343

    Purpose

    for Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

    Depositor

    Cheng-Yu Lee

    Article

    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    mCherry::Renilla(nls)

    Expression

    Insect

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. LB124 (Bcl-2 from -1287 to -3934)

    Plasmid

    #15240

    Depositor

    Linda Boxer

    Article

    Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

    Insert

    B-cell lymphoma/leukemia-2 (BCL2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Bacterial

    Mutation

    Bcl-2 fragment from -1287 to -3934 with P1

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

    Plasmid

    #136513

    Purpose

    Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

    Insert

    codon-optimized AcrIIA5
    

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Src(WT)-SsrA(Y7I)-mCer3

    Plasmid

    #220998

    Purpose

    This is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminal

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA, mCerulean3 (Src Mouse)
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Y7I in SsrA

    Promoter

    CMV

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATP1A1_G6_T804N_Dual_pegRNA

    Plasmid

    #173200

    Purpose

    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N pegRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. DCT.VV.Orf40

    Plasmid

    #12728

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf40
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pMXs-Etv3

    Plasmid

    #131610

    Purpose

    expression of mouse Etv3 in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Etv3 (Etv3 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. binder/tag-WT-FAK-insertionB

    Plasmid

    #190440

    Purpose

    The tag is SsrA (AANDENY). Tag is inserted after D405 of FAK.

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    FAK (Ptk2 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SsrA inserted after aa 405

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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