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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,943 results for: grna

Showing: 10021 - 10040 of 15943 results

  3. pSECRETS-A

    Plasmid
    #196986
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    S. pyogenes Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pltetO1
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196986-plasmid-map-sequence-id-396953

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  5. pMZ374

    Plasmid
    #59896
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59896-plasmid-map-sequence-id-313658

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  6. pCfB3405

    Plasmid
    #106166
    Purpose
    EasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106166-plasmid-map-sequence-id-206142

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  9. pCFD5-NS

    Plasmid
    #149546
    Purpose
    Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Article
    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    149546-plasmid-map-sequence-id-306364

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  12. pRGEB33

    Plasmid
    #158151
    Purpose
    Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.
    Depositor
    Kabin Xie
    Article
    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    158151-plasmid-map-sequence-id-320624

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  17. pCR-Hyg

    Plasmid
    #158708
    Purpose
    For cloning and constitutive expression of crRNAs in mycobacterium
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    crRNA
    Use
    CRISPR and Synthetic Biology; Mycobacteria expres…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Hsp60
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158708-plasmid-map-sequence-id-320872

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Showing: 10021 - 10040 of 15943 results