We narrowed to 31,231 results for: his

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pTF276 (pFA6a-PSc-6xGly-2xStrep-His3MX)

Plasmid

#44064

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pTF266 (pFA6a-PSc-6xGly-3xFlag-His3MX)

Plasmid

#44065

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

3xFlag, 6xGly linker, ADH1 transcription terminat…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pNIC-CFP

Plasmid

#173074

Purpose

Expression vector for E. coli producing target protein with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Tags

mCerulean

Expression

Bacterial

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pPD189 VP16-ZF37

Plasmid

#138759

Purpose

Constitutive expression of VP16-ZF37 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF37

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLN599 (pFuGW-hUbCp-hSTE1)

Plasmid

#105206

Purpose

ubiquitin C promoter drives STE expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

hUbCp-hSTE1

Use

Lentiviral and Synthetic Biology

Tags

HA tag

Expression

Mammalian

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLN466 (FuGW-S(cMYC)p-mKate2)

Plasmid

#105189

Purpose

synthetic promoter cMYC drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(cMYC)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLN351 (FuGW-S(YBX1)p-mKate2 )

Plasmid

#105174

Purpose

synthetic promoter YBX1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(YBX1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLN352 (FuGW-S(Elk1)p-mKate2)

Plasmid

#105175

Purpose

synthetic promoter Elk1 drives mKate2 experssion

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(Elk1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

Plasmid

#159127

Purpose

expresses FBXO22 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

FBXO22 (FBXO22 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1_F734Y-hDPP9_6His

Plasmid

#254905

Purpose

pFastBac1 vector for the recombinant overexpression in insect cells of the human DPP9 protein mutated at position 734.

Depositor

Ingrid De Meester

Article

Beyens et al ChemMedChem. 2025 Mar 3;20(5):e202400700

Insert

Dipeptidyl peptidase 9 (DPP9 Human)

Tags

6His-tag

Expression

Insect

Mutation

Changed Phenylalanine 734 to Tyrosine.

Promoter

Polyhedrin

Available Since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-polyubiquitin_6xHis

Plasmid

#256080

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Article

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Polyubiquitin

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-microtubuleAP_6xHis

Plasmid

#256081

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Article

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Microtubule-associated protein

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-8HisG2K

Plasmid

#225540

Purpose

Empty backbone for bacterial expression. 8xHis tag, K in position 2 to minimise posttranslational gluconoylation.

Depositor

Gregory Challis

Type

Empty backbone

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Soluble CD20His

Plasmid

#246596

Purpose

Computationally designed water-soluble mimic of the transmembrane protein CD20 dimer. For expression as secreted protein in Expi293 cell. Insert: HSA-his8-TEVsolubleCD20- (10.4kDa)

Depositor

Brian Kuhlman

Article

Yao et al Protein Sci. 2025 Aug;34(8):e70184. doi:

Insert

Soluble CD20

Tags

HSA-8xHis-TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHS26_10xHis_MBP_TEV_VRER-Cas9

Plasmid

#244829

Purpose

Bacterial expression of VRER Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

VRER Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135V/G1218R/R1335E/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS23_10xHis_MBP_TEV_SpG-Cas9

Plasmid

#244826

Purpose

Bacterial expression of SpG Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS24_10xHis_MBP_TEV_SpRY-Cas9

Plasmid

#244827

Purpose

Bacterial expression of SpRY Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS25_10xHis_MBP_TEV_EQR-Cas9

Plasmid

#244828

Purpose

Bacterial expression of EQR Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

EQR Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135E/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS73_10xHis_MBP_TEV_SpRY-Cas9_2xNLS

Plasmid

#244835

Purpose

Bacterial expression of SpRY Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-HIS3-3

Plasmid

#232882

Purpose

Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

HIS3 gRNA (HIS3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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