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EZH2-TALEN_Right_No_FLAG

Plasmid

#79900

Purpose

Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Publication

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Right_No_FLAG (EZH2 Human)

Use

TALEN

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTU1-B-RFP

Plasmid

#72940

Purpose

Level 1 Destination vector - Position B

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - B site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-148

Plasmid

#68749

Purpose

ZF8/ZF7-TF with mCherry marker

Depositor

James Collins

Publication

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF8/ZF7-VP64
mCherry

Tags

NLS and flag

Expression

Mammalian

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-149

Plasmid

#68750

Purpose

ZF6/ZF10-TF with mCherry marker

Depositor

James Collins

Publication

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF6/ZF10-VP64
mCherry

Tags

NLS and flag

Expression

Mammalian

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x5

Plasmid

#63599

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Publication

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Snail1-3'UTR

Plasmid

#61781

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR

Use

Luciferase

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
LI B-C T-Saphire

Plasmid

#54424

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

T-saphhire + 16 aa GS linker

Use

Cloning vector

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C mOrange

Plasmid

#54427

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mOrange + 16 aa GS linker

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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NovoSplit657(B)-mNeonGreen

Plasmid

#255885

Purpose

Expression plasmid for NovoSplit657(B), the second part of the NovoSplit system, fused to mNeonGreen for fluorescence labeling.

Depositor

Julia Mahamid

Publication

Tran et al Science. 2026 Jul 16:eaeb0822. doi: 10.1

Insert

NovoSplit657(B)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJB127

Plasmid

#248630

Purpose

Theophylline-based control of repA on a C. difficile plasmid for use in allelic exchange and codA added to help cure strains of the plasmid.

Depositor

Joseph Sorg

Publication

Brehm et al Anaerobe. 2024 Apr 29;88:102858. doi: 10

Inserts

sacB (sacB )
codA

Use

Other

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFP

Plasmid

#244210

Purpose

Mammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Sc [Pol31 + Pol32]-pRS403/GAL

Plasmid

#239199

Purpose

Overerxpress Sc Pol31 & Pol32 when integrated into yeast (S. cer)

Depositor

Michael O'Donnell

Inserts

Pol31 (POL31 Budding Yeast)
Pol32 (POL32 Budding Yeast)

Expression

Yeast

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

Plasmid

#229828

Purpose

A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SisTnpB1–ωRNA

Plasmid

#228365

Purpose

Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

Depositor

Tao Liu

Publication

Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

Insert

SisTnpB1

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGLucIPZ

Plasmid

#228703

Purpose

Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Publication

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Gaussia luciferase
non-silencing shRNA

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTn7-SCOUT21.RY

Plasmid

#201006

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT22.RY

Plasmid

#201018

Purpose

pLv1-pUC18R6KT-miniTn7-Km-mCherry-sYFP2, KmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLRD14

Plasmid

#208266

Purpose

Production of syn-pimara-7,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL4

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0017

Plasmid

#205586

Purpose

pNpuDnaE(C)_HygR(90-341)_3HA

Depositor

Xiaohan Yang

Publication

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NpuDnaE(C)-HygR

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHLP701

Plasmid

#203743

Purpose

C-terminal tagging of target proteins with 3xV5-IAA17 degron

Depositor

Mark Hall

Publication

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

IAA17 auxin binding domain

Use

Pcr amplification template for gene tagging

Tags

3xV5

Promoter

none

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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