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pU6-sgCD71-2

Plasmid

#46918

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgCD71 -2 (TFRC Human)
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-At-TRL

Plasmid

#32242

Depositor

Stan Fields, Jay Hesselberth

Publication

Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

Insert

A. thaliana tRNA ligase (RNL Mustard Weed)

Tags

6xHistidine

Expression

Bacterial

Promoter

T7 RNA Polmyerase

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMIEG3-BCL6

Plasmid

#40339

Depositor

Alexander Dent

Publication

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

BCL6 (BCL6 Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

optimized translation initiation codon

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMXs-IP-EGFP-hAtg13

Plasmid

#38191

Depositor

Noboru Mizushima

Publication

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
PKC iota CAT

Plasmid

#21254

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC iota (PRKCI Human)

Expression

Mammalian

Mutation

delta 1-231

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
mGluR6 (200bp crit reg)+SV40p-GFP-IRES-AP

Plasmid

#18817

Depositor

Connie Cepko

Publication

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

mGluR6 (200bp crit reg)+SV40p-GFP-IRES-AP

Expression

Mammalian

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLTR-RVG

Plasmid

#17577

Depositor

Jakob Reiser

Publication

Mochizuki et al J Virol. 1998 Nov . 72(11):8873-83.

Insert

rabies virus glycoprotein (SAD B19 strain)

Use

Lentiviral

Expression

Mammalian

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LPCX Smad2 deltaSSMS

Plasmid

#12637

Depositor

Rik Derynck

Publication

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

Smad2 (SMAD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Last 4 aa's deleted.

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
HA.PKD.S916A

Plasmid

#10817

Depositor

Alex Toker

Publication

Storz et al Mol Pharmacol. 2004 Oct . 66(4):870-9.

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

S910A--active; L520P

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
His-Tev-UbE1

Plasmid

#186804

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

Depositor

Lalit Deshmukh

Publication

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-UbE1 (UBA1 Human)

Tags

His6-Tev

Expression

Bacterial

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-PLIN3

Plasmid

#161918

Purpose

Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Publication

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Perilipin-3 (PLIN3 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-SOX4

Plasmid

#110360

Purpose

Mammalian expression of FLAG-tagged SOX4

Depositor

Carlos Moreno

Publication

Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

Insert

SOX4 (SOX4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

lacking 5' UTR

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-BioID-FAM21

Plasmid

#121046

Purpose

Expresses GFP tagged BioID and human FAM21C in mammalian cells

Depositor

Gia Voeltz

Publication

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Inserts

BioID
FAM21C (FAM21C Human)

Tags

GFP

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

CMV

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pADSL-EGFP

Plasmid

#99109

Purpose

Expresses human ADSL-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Publication

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

ADSL (ADSL Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTH2

Plasmid

#84452

Purpose

shuttle plasmid for sarA P1-CFP expression

Depositor

Reindert Nijland

Publication

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

CFP Cerulean

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX462-hPRKAA1-gRNA_B

Plasmid

#74375

Purpose

gRNA_B to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Publication

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p306-BrdU-Inc

Plasmid

#71792

Purpose

BrdU-incorporating plasmid with selectable marker URA3

Depositor

Oscar Aparicio

Publication

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pC4-RhE-FRB-Fis1

Plasmid

#68056

Purpose

express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

Depositor

Richard Youle

Publication

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

Fis1 (FIS1 Human)

Tags

FRB

Expression

Mammalian

Mutation

Fis1 tail (aa 93-152)

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVE-hTGFB3-IRES-Red

Plasmid

#52580

Purpose

2nd generation lentiviral transfer vector driving expression of human TGF-beta3 and RFP in mammalian cells

Depositor

Charles Gersbach

Publication

Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

human transforming growth factor-beta3 (TGFB3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEMABC

Plasmid

#45398

Depositor

Katsu Murakami

Publication

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Inserts

rpoA (rpoA Escherichia coli)
rpoB (rpoB Escherichia coli)
rpoC (rpoC Escherichia coli)

Expression

Bacterial

Promoter

None and T7

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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