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pHR-PGK-LexA-WW-CIB1-biLINuS

Plasmid

#171757

Purpose

Lentiviral vector for constitutive expression of fusion protein LexA-WW-CIB1-biLINuS

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

Synthetic gene containing LexA-WW-CIB1-biLINuS

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase

Plasmid

#163404

Purpose

Expresses BMPR-2 lacking the kinase domain in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#2-mCherry

Plasmid

#164097

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp7-EGFP

Plasmid

#165111

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp10-mCherry

Plasmid

#165135

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp10 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-NpHR3.3-EYFP

Plasmid

#137151

Purpose

Viral expression of NpHR3.3-EYFP

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

NpHR3.3-EYFP

Use

AAV

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM18

Plasmid

#135484

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-tdTomato ADH1t

Tags

tdTomato

Expression

Bacterial and Yeast

Promoter

SpunH2Bpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1g

Plasmid

#134991

Purpose

Expression of mouse Myo 1g in mammalian cells.

Depositor

Martin Bähler

Article

Olety et al FEBS Lett. 2010 Feb 5;584(3):493-9. doi:

Insert

Myo 1g (Myo1g Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRA

Plasmid

#131035

Purpose

Expresses OT-I TCRA

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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FEV_pet28a

Plasmid

#131668

Purpose

Bacterial expression of ETS gene FEV

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

FEV (FEV Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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ETV5_pet28a

Plasmid

#131639

Purpose

Bacterial expression of ETS gene ETV5

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV5 (ETV5 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-myc

Plasmid

#109422

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope

Tags

BAP, HA, His6, T7, and myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-Dronpa

Plasmid

#87709

Purpose

FKBP-tagged Dronpa.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FKBP-Dronpa

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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p7128 pHAGE-P-CMVt-N-HA-GAW-HLA-A

Plasmid

#100154

Purpose

Expresses HLA-A

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HLA-A (HLA-A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

Plasmid

#86414

Purpose

nuclear split GFP11 reporter without mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-PUS1

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1pr

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTol-Promoter:NLS-tdMCP-eGFP

Plasmid

#86248

Purpose

For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Insert

beta Actin promoter:NLS-tdMCPeGFP

Use

Used with transposase to make transient or stable…

Tags

NLS-tdMCP-eGFP

Promoter

Beta Actin

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo

Plasmid

#80032

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Article

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-338

Plasmid

#68795

Purpose

Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2

Tags

IRES, NLS, flag, and nls

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-tagBFP

Plasmid

#65257

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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