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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,013 results for: flag

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Showing: 10061 - 10080 of 15013 results

  3. pKAR1

    Plasmid
    #23104
    Purpose
    Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid
    Depositor
    Randy Poon
    Publication
    Ma et al Nucleic Acids Res. 2007 . 35(4):e22.
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    TRE for cDNA; U6 for shRNA
    Available since
    May 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    23104-plasmid-map-sequence-id-10551

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  14. pmiCas9

    Plasmid
    #162758
    Purpose
    express miCas9
    Depositor
    Eugene Chen
    Publication
    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miCas9
    Use
    CRISPR
    Tags
    FLAG and NLS
    Expression
    Mammalian
    Mutation
    Contains Brex27 sequence range is 4958-5065
    Promoter
    CMV
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    162758-plasmid-map-sequence-id-329432

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  15. pYPQ266E

    Plasmid
    #161521
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing
    Depositor
    Yiping Qi
    Publication
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161521-plasmid-map-sequence-id-325638

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  16. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid
    #159086
    Purpose
    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.
    Depositor
    Jeremy Day
    Publication
    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589
    Insert
    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    EGFP and FLAG
    Expression
    Mammalian
    Promoter
    hSYN, U6
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159086-plasmid-map-sequence-id-310691

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  18. pCMV-BE3-FNLS(RA)

    Plasmid
    #112671
    Purpose
    CMV expression vector for BE3-FNLS construct (codon optimized)
    Depositor
    Lukas Dow
    Publication
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-FNLS
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    NLS sequence at the N-terminus and D10A
    Promoter
    CMV
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112671-plasmid-map-sequence-id-217194

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  19. pIRES-hrGFP II-mTET1

    Plasmid
    #83569
    Purpose
    Expresses TET1 with a mutated catalytic domain in mammalian cells
    Depositor
    Jean-Pierre Issa
    Publication
    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    Tags
    3X FLAG tag and hr GFP II
    Expression
    Mammalian
    Mutation
    catalytic domain mutant H1672D, D1674A
    Promoter
    CMV
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83569-plasmid-map-sequence-id-191033

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Showing: 10061 - 10080 of 15013 results