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EF1a_ONECUT1_P2A_Hygro_Barcode

Plasmid

#120470

Purpose

Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ONECUT1 (ONECUT1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ERG_P2A_Hygro_Barcode

Plasmid

#120434

Purpose

Barcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ERG (ERG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:WTIP

Plasmid

#108231

Purpose

Lentiviral vector for Tet-inducible WTIP expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

WTIP (WTIP Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-HITI

Plasmid

#87117

Purpose

AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA

Use

AAV and CRISPR

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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p005kanRFP

Plasmid

#58533

Purpose

RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Red fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5 between two lac operators

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe-GW-HA-RINT1

Plasmid

#39841

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

RINT1 (RINT1 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt5A STOP

Plasmid

#35874

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-6HA

Plasmid

#18035

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-6 (Wnt6 Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_scFV-sfGFP-G9aCD

Plasmid

#235580

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector G9a fused with scFV to programme H3K9me2/3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

G9a (Ehmt2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ETV6_RUNX1

Plasmid

#205818

Purpose

Express mEGFP-tagged fusion protein, ETV6_RUNX1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ETV6_RUNX1 (ETV6, RUNX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_SST1.0

Plasmid

#208665

Purpose

Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based somatostatin (SST) sensor GRAB_SST1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2971

Plasmid

#144447

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR1H4 (NR1H4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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U6-pegRNA-H1-nick sgRNA-mCherry

Plasmid

#176901

Purpose

Expresses prime editing guide RNA and guide RNA in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

mCherry
gRNA scaffold
gRNA scaffold

Use

AAV

Expression

Mammalian

Promoter

CAG, H1, and U6

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDF0336 pAAV EFS-HvsCas7-11 for dual vector

Plasmid

#172515

Purpose

Encodes HvsCas7-11 mammalian expression casette in AAV backbone

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV EFS-HvsCas7-11 for dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307-IBV-3CL

Plasmid

#160288

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-VPR-2AmScarlet

Plasmid

#121964

Purpose

Mammalian expression, Transcription regulation module

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

VP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_NRL_P2A_Hygro_Barcode

Plasmid

#120469

Purpose

Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NRL (NRL Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1 Aquamarine

Plasmid

#106285

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Aquamarine

Tags

6xHis and TEV cleavage (N terminal on insert)

Expression

Bacterial

Mutation

T65S and H148G

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRFP-N1

Plasmid

#108409

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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