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pet23a_RBPMS_20_101

Plasmid

#65733

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 20-101

Promoter

T7

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_1_101

Plasmid

#65728

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-101

Promoter

T7

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_1_120

Plasmid

#65730

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-120

Promoter

T7

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR4-RBPMS

Plasmid

#59387

Purpose

Entry vector with RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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psicheck-2-LPPRC

Plasmid

#48167

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

LRPPRC leucine rich pentatricopeptide repeat containing (Lrpprc Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pEMS1102

Plasmid

#29033

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748)

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple183 (S100B Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago Chi-d

Plasmid

#14087

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-d

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-d

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pIND4-RC-M210TAG-A7

Plasmid

#223015

Purpose

Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for 3-nitrotyrosine

Depositor

Steven Boxer

Inserts

puhA
pufL
pufM
A7 RS
pylT tRNA

Tags

6xHis

Mutation

changed Tyr M210 to amber stop codon (TAG) for am…

Promoter

rrnB

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKT-CNP-inact

Plasmid

#211804

Purpose

Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

Depositor

Emily Weinert

Article

Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

Insert

2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)

Expression

Bacterial

Mutation

H73L H153L

Promoter

tet

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pOPO636

Plasmid

#195299

Purpose

pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

TnsABCQ operon of McCAST (Tn7575)

Expression

Bacterial

Promoter

IPTG inducible lac promoter

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (SMAD4)

Plasmid

#180191

Purpose

AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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secCitrine

Plasmid

#181964

Purpose

Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

signal peptide sequence from basic chitinase

Tags

Citrine YFP

Expression

Plant

Promoter

1.5 kb MUM4 promoter

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_Rat_cebp-alfa

Plasmid

#140098

Purpose

CRISPR-Cas9 library validation (positive control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_SPI1

Plasmid

#140099

Purpose

CRISPR-Cas9 library validation (positive control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P1-N-EGFP-C

Plasmid

#159436

Purpose

CRISPR knock-in donor construct with fluorescent reporter

Depositor

Jianhua Xing

Article

Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

Insert

EGFP

Use

CRISPR

Tags

3' linker pair 1, 5' linker pair 1, C t…

Promoter

CMV

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-ATP5O

Plasmid

#139842

Purpose

Lentiviral expression of human ATP5O

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

ATP5O (ATP5PO Human)

Use

Lentiviral

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestRFP_HuC

Plasmid

#65765

Purpose

expresses RFP-tagged HuC in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL3 (ELAVL3 Human)

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Unique to HeLa

Pooled Library

#86545

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - U87 & HEK293T

Pooled Library

#86547

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only