We narrowed to 36,793 results for: KAN

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pET28a-HsSENP5

Plasmid

#71468

Purpose

Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 5 (SENP5 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
mCitrine-MAP4-C-10

Plasmid

#56382

Purpose

Localization: Microtubules, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Insert

MAP4

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pOPTN1-424-EGFP

Plasmid

#68840

Purpose

deleted OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-424 only

Promoter

CMV

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
PAX6 sgRNA7

Plasmid

#68466

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCB52

Plasmid

#69747

Purpose

Retroviral expression of Cyclin B1

Depositor

Iain Cheeseman

Insert

Cyclin B1 (CCNB1 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-PLK4-S305E-3xFLAG

Plasmid

#69840

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305E

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-345

Plasmid

#68785

Purpose

R->A Sensor pair 1 with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V2 R-->A mutant
N-Terminal Sensor 1 V2 R-->A mutant
mCherry

Tags

NLS, flag, and nls

Expression

Mammalian

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pOPTN1-55-EGFP

Plasmid

#68838

Purpose

deleted OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-55 only

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pEGB Pnos:Luciferase:Tnos (GB0112)

Plasmid

#68217

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-338

Plasmid

#68795

Purpose

Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2

Tags

IRES, NLS, flag, and nls

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV2_VP1_RaPID_r1c3

Plasmid

#65723

Purpose

Produce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 only

Depositor

Junghae Suh

Article

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

mCherry

Use

AAV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-PTRF

Plasmid

#68401

Purpose

Expression of fluorescently tagged PTRF in mammalian cells

Depositor

Rob Parton

Article

Hill et al Cell. 2008 Jan 11;132(1):113-24. doi: 10

Insert

PTRF (Cavin1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF4-Myc

Plasmid

#67350

Purpose

Mammalian expression of hArf4 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Tags

Myc

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF5-Myc

Plasmid

#67351

Purpose

Mammalian expression of hArf5 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF1-Myc

Plasmid

#67348

Purpose

Mammalian expression of hArf1 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pT7oGFP

Plasmid

#69346

Purpose

Orthogonal GFP message under T7 promoter

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

Super folder GFP

Use

Unspecified

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLpp5oGFP

Plasmid

#69345

Purpose

Orthogonal GFP message under lpp5 promoter

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

super folder GFP

Expression

Bacterial

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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p42.3A7

Plasmid

#69575

Purpose

OT-I TCR a chain cDNA in pCR1000

Depositor

Francis R. Carbone

Article

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR alpha clone 149.42 (Tcra Mouse)

Expression

Bacterial

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR3c GW CHD5

Plasmid

#68869

Purpose

GW entry vector for CHD5

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Gateway Cloning

Promoter

none

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
Kohinoor-Clathrin

Plasmid

#67773

Purpose

Clathrin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Clathrin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only