We narrowed to 11,585 results for: Vars;

1. pGP-AAV-syn-iGABASnFR2-WPRE

   Plasmid

   #218874

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   Synapsin

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDACR3883 [rab-3p::HYlight-RA::let-858utr]

   Plasmid

   #207667

   Purpose

   Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

   Depositor

   Daniel Colón-Ramos

   Article

   Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   HYlight-RA [T152E]
   

   Expression

   Worm

   Promoter

   rab-3

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. bdSUMO-ZF5.3-GFP-HiBit

   Plasmid

   #170992

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. bdSUMO-HA2-GFP-HiBit

   Plasmid

   #171017

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-HA2-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJR031

   Plasmid

   #198825

   Purpose

   Stable step function ChR2 variant. Activated by bluelight, terminated by green light.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJR032

   Plasmid

   #198773

   Purpose

   Stabilized step function Opsins ChR2 variant

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::hChR2 (C128S, D156A)-EYFP::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p221a-GUS

   Plasmid

   #71263

   Purpose

   Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   Beta-glucuronidase
   

   Use

   Gateway

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. F-IRF7

   Plasmid

   #169777

   Purpose

   Express IRF7 in mammalian cells

   Depositor

   Sung-Liang Yu

   Article

   Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

   Insert

   interferon regulatory factor 7 (IRF7), transcript variant a (IRF7 Human)
   

   Tags

   Flag tag

   Expression

   Bacterial and Mammalian

   Available Since

   July 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Syn.GCaMP6f.WPRE.SV40

   Plasmid

   #100837

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a hMDH1v3 (+TEV)

    Plasmid

    #204229

    Purpose

    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH1v3 (MDH1 Human)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Inserted a G after ATG to maintain kozak site

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSKA413

    Plasmid

    #80381

    Purpose

    Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    ccaS (complement)
    ccaR (complement)
    gfpmut3
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    FLAG tag

    Expression

    Bacterial

    Promoter

    ccaR and cpcG2Δ59

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1+DPP6

    Plasmid

    #117272

    Purpose

    Encodes human DPP6 variant 1

    Depositor

    Mark Cannell

    Article

    Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

    Insert

    DPP6 (DPP6 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.Syn.GCaMP6s.WPRE.SV40

    Plasmid

    #100843

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-CAG-flex-iGABASnFR2-WPRE

    Plasmid

    #218880

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-sbGLuc-B7-EYFP

    Plasmid

    #114110

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.

    Inserts

    Gaussia luciferase, slow burn
    B7 hinge region
    Enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid

    #110555

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    Anti-RD114 heavy chain
    

    Tags

    mIgG2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

    Plasmid

    #110556

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    anit-RD114 light chain
    

    Tags

    mKappa

    Expression

    Bacterial and Mammalian

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

    Plasmid

    #52521

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Use

    AAV and Cre/Lox

    Tags

    2xHA on ChR2

    Promoter

    CBA

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid

    #134470

    Purpose

    Human expression vector for SaCas9-HF variant.

    Depositor

    Zongli Zheng

    Article

    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

    Insert

    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    

    Use

    CRISPR

    Tags

    SV40NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    R245A, N413A, N419A, R654A

    Promoter

    CAG

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAF0034 Ef1a-Kp03-2a-EGFP

    Plasmid

    #193461

    Purpose

    Expression of Kp03 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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