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Showing: 10141 - 10160 of 18429 results

  1. gWiz_BM40-vAchT-Cytosolic-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218446
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter
    Depositor
    Axel Brunger
    Insert
    vAchT-Cytosolic-Vh-hIgG1-CH6
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218446-plasmid-map-sequence-id-434499

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  2. GBH-2aG4-gcryBFP.3

    Plasmid
    #188015
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188015-plasmid-map-sequence-id-380747

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  3. P14-pZE-SUMO-ObiH

    Plasmid
    #204629
    Purpose
    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized obiH gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
    Depositor
    Aditya Kunjapur
    Article
    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:
    Insert
    His-SUMO-TEV-ObiH
    Use
    Synthetic Biology
    Tags
    His Tag; SUMO Tag; TEV Protease Site
    Expression
    Bacterial
    Promoter
    PLtetO-1 promoter
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204629-plasmid-map-sequence-id-414418

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  6. B-HiFi SpCas9

    Plasmid
    #207362
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HiFi SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    R691A, amino acids 1005-1013 replaced with two gl…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207362-plasmid-map-sequence-id-410135

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  9. pGEX6P1 pS43 METTL3

    Plasmid
    #200732
    Purpose
    Bacterial expression of GST-Tagged codon optimized human pS43 METTL3
    Depositor
    Claudio Alarcón
    Insert
    pS43 METTL3 (METTL3 Human)
    Tags
    GST from backbone plasmid
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli, UAG co…
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200732-plasmid-map-sequence-id-401809

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  10. pCI_mini-mAgrin-mSA-Myc-His

    Plasmid
    #198135
    Purpose
    Express mini-Agrin in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    mini-Agrin (Agrn Mouse)
    Tags
    mSA, Myc-His
    Expression
    Mammalian
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available Since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198135-plasmid-map-sequence-id-393454

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  11. p663-UBC-miniTurbo-V5-NEK3_IDG-K

    Plasmid
    #198006
    Purpose
    Gateway destination clone of NEK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-NEK3 (NEK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    198006-plasmid-map-sequence-id-390032

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  12. p663-UBC-miniTurbo-V5-MAST3_IDG-K

    Plasmid
    #198007
    Purpose
    Gateway destination clone of MAST3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-MAST3 (MAST3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    198007-plasmid-map-sequence-id-390026

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  13. FUG-P2A-tC3-W

    Plasmid
    #129475
    Purpose
    3rd-generation lentiviral vector for hUbC-driven co-expression of EGFP and truncated C3
    Depositor
    Robert Gross
    Insert
    truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    human ubiquitin C
    Available Since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    129475-plasmid-map-sequence-id-384588

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  14. pDEST27-GST-hADAM10

    Plasmid
    #189553
    Purpose
    Protein expression ADAM10 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    GST
    Expression
    Mammalian
    Available Since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    189553-plasmid-map-sequence-id-384138

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  15. hSyn-Aion-ts-mScarlet-ER

    Plasmid
    #192577
    Purpose
    AAV-vector for optogenetic long-term silencing of neurons
    Depositor
    Simon Wiegert
    Insert
    Aion
    Use
    AAV
    Tags
    ts-mScarlet-ER
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    human synapsin
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192577-plasmid-map-sequence-id-379289

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  16. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid
    #192585
    Purpose
    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    Lentiviral
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Promoter
    Ef1-alpha
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192585-plasmid-map-sequence-id-379281

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  17. p667-UBC-CLK4-V5-miniTurbo_IDG-K

    Plasmid
    #189913
    Purpose
    Gateway destination clone of CLK4 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CLK4 (CLK4 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    189913-plasmid-map-sequence-id-374023

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  18. pIVT-myc-T58A-S62D

    Plasmid
    #138540
    Purpose
    For making myc-T58A-S62D RNA
    Depositor
    Carmen Williams
    Insert
    Myelocytomatosis oncogene T58A S62D (Myc Mouse)
    Use
    In vitro transcription
    Mutation
    Threonine 58 changed to alanine and serine 62 cha…
    Promoter
    T7
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    138540-plasmid-map-sequence-id-291746

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  19. pcDNA5-FRT-hMYC

    Plasmid
    #185373
    Purpose
    For mammalian expression of human MYC
    Depositor
    Ayaz Najafov
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    185373-plasmid-map-sequence-id-363449

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  20. pcDNA3-CYP450-μDpTT

    Plasmid
    #182325
    Purpose
    Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DAKAP-μDronpa-TagRFP-T
    Tags
    N-terminal 27 amino acids from CYP450
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182325-plasmid-map-sequence-id-362816

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Showing: 10141 - 10160 of 18429 results