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Plasmid#227774PurposeMiddle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloningDepositorInsertloxP-dsR-loxP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pBsVchDonor
Plasmid#203814PurposeDNA donor for transpositionDepositorInsertMini-Tn, aprE cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM315 pACYC-His6-Rpn10[ΔUIM]
Plasmid#226348PurposeExpression of yeast Rpn10 with the UIM deletedDepositorInsertrpn10 (RPN10 Budding Yeast)
UseTagsHisExpressionBacterialMutationdelta UIMPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAG66
Plasmid#226751PurposeExpresses exogenous GFPDepositorInsertGFP
UseLentiviralTagssfGFPExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a_SMOX
Plasmid#186345PurposeC-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cellsDepositorInsertSpermine oxidase (SMOX Human)
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB
Plasmid#225064PurposeExpression of HalM-modified HalA2 in human cellsDepositorInsertHalA2
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationWTPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLigify_dRibose
Plasmid#215561PurposeExpresses GFP in response to D-RiboseDepositorInsertsGFP mut2
LcLacI
UseTagsExpressionBacterialMutationPromoterP250 and PlclaciAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYGS Q16C
Plasmid#217674PurposeFor expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experimentDepositorInsertCRYGS (CRYGS Human)
UseTags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to CysteinePromoterT7Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS8
Plasmid#218716PurposeExpresses N-terminally EGFP-tagged BBS8 in mammalian cellsDepositorInsertBBS8 (TTC8 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3 Ras-LOCKR-PL Key
Plasmid#216503PurposeRas activity dependent proximity labeler, Key only, untargetedDepositorInsertRas-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-PL Cage
Plasmid#216502PurposeRas activity dependent proximity labeler, Cage only, untargetedDepositorInsertRas-LOCKR-PL Cage
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PM-Ras-LOCKR-PL Key
Plasmid#216504PurposeRas activity dependent proximity labeler, Key only, plasma membrane localizedDepositorInsertPM-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Golgi-Ras-LOCKR-PL Key
Plasmid#216505PurposeRas activity dependent proximity labeler, Key only, Golgi localizedDepositorInsertGolgi-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 ER-Ras-LOCKR-PL Key
Plasmid#216506PurposeRas activity dependent proximity labeler, Key only, ER localizedDepositorInsertER-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Mito-Ras-LOCKR-PL Key
Plasmid#216507PurposeRas activity dependent proximity labeler, Key only, mitochondria localizedDepositorInsertMito-Ras-LOCKR-PL
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY380
Plasmid#182962PurposeAmp-resistant, low copy (p15A ori), E. coli 5'ptsI transcriptionally fused with GFP.DepositorInsertptsI
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY390
Plasmid#182963PurposeAmp-resistant, low copy (p15A ori), E. coli ptsI transcriptionally fused with GFP.DepositorInsertptsI
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 AvrRpm1
Plasmid#209185PurposepENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tagsDepositorInsertAvrRpm1
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2A
Plasmid#195707PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2A domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 124-211 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2B
Plasmid#195708PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2B domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 253-344 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2AB
Plasmid#195709PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2AB domainDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 104-386 of mouse SYT9PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2A
Plasmid#195710PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2A domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-264 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2B
Plasmid#195711PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2B domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 248-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2AB
Plasmid#195712PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2AB domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET (SUMO)-Syt9
Plasmid#195713PurposeBacterial expression plasmid encoding SUMO-tagged full-length Syt9DepositorInsertSyt9 (Syt5 Rat)
UseTagsGST-SUMOExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgVchcrRNA
Plasmid#203815PurposeExpression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertCRISPR(BsaI)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ4
Plasmid#198176PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 μ4
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W390A_P
Plasmid#147236PurposeInsect Expression of CeAIN2-W390ADepositorInsertCeAIN2-W390A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN1_1-504_P
Plasmid#147238PurposeInsect Expression of CeAIN1_1-504DepositorInsertCeAIN1_1-504 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeNot1_P
Plasmid#147239PurposeInsect Expression of CeNot1DepositorInsertCeNot1
UseTagsExpressionInsectMutationone non silent mutation A610T compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CePAN3_1-244_P
Plasmid#147243PurposeInsect Expression of CePAN3_1-244DepositorInsertCePAN3_1-244
UseTagsExpressionInsectMutationInsertion of two AA (FQ) at position 172 compared…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-HsDCP1a_1-135_P
Plasmid#147246PurposeBacterial Expression of HsDCP1_1-135DepositorInsertHsDCP1_1-135
UseTagsExpressionBacterialMutationone silent mutation T237C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpHN-HsXRN1_1650-1706_P
Plasmid#147247PurposeBacterial Expression of HsXRN1_1650-1706DepositorInsertHsXRN1_1650-1706 (XRN1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1630-1690_P
Plasmid#147249PurposeMammalian Expression of HsXRN1_1630-1690DepositorInsertHsXRN1_1630-1690 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN1_1-504_P
Plasmid#147227PurposeInsect Expression of CeAIN1_1-504DepositorInsertCeAIN1_1-504 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN1_351-460_P
Plasmid#147228PurposeInsect Expression of CeAIN1_351-460DepositorInsertCeAIN1_351-460 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN1_351-504_P
Plasmid#147229PurposeInsect Expression of CeAIN1_351-504DepositorInsertCeAIN1_351-504 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN1-del351-504_P
Plasmid#147230PurposeInsect Expression of CeAIN1-del351-504DepositorInsertCeAIN1-del351-504 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-3xWmut_P
Plasmid#147231PurposeInsect Expression of CeAIN2-3xWmutDepositorInsertCeAIN2-3xWmut
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-del254-400_P
Plasmid#147232PurposeInsect Expression of CeAIN2-del254-400DepositorInsertCeAIN2-del254-400
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W282AW366A_P
Plasmid#147233PurposeInsect Expression of CeAIN2-W282AW366ADepositorInsertCeAIN2-W282AW366A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2-W282AW390A_P
Plasmid#147234PurposeInsect Expression of CeAIN2-W282AW390ADepositorInsertCeAIN2-W282AW390A
UseTagsExpressionInsectMutationDeletion of two AA SD 378-379 compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W282AW390A_O
Plasmid#147143PurposeInsect Expression of CeAIN2-W282AW390ADepositorInsertCeAIN2-W282AW390A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W366A_O
Plasmid#147144PurposeInsect Expression of CeAIN2-W366ADepositorInsertCeAIN2-W366A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W366AW390A_O
Plasmid#147145PurposeInsect Expression of CeAIN2-W366AW390ADepositorInsertCeAIN2-W366AW390A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeAIN2-W390A_O
Plasmid#147146PurposeInsect Expression of CeAIN2-W390ADepositorInsertCeAIN2-W390A
UseTagsExpressionInsectMutationDeletion of 2 AA SD at position 378-379 compared …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN1_351-641_O
Plasmid#147123PurposeInsect Expression of CeAIN1_351-641DepositorInsertCeAIN1_351-641 (ain-1 Nematode)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only