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C321.∆A ompT- rne- endA- lon-

Bacterial Strain

#182775

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne-

Bacterial Strain

#182777

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A ompT- rne- endA-

Bacterial Strain

#182774

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A ompT- rne- lon-

Bacterial Strain

#182773

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
pIMAY

Plasmid

#68939

Purpose

E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

Depositor

Tim Foster

Article

Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

Type

Empty backbone

Use

Staphylococcal allelic exchange vector

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Article

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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px330 SUZ12 - pDY083

Plasmid

#171109

Purpose

Expresses Cas9 and an sgRNA targeting SUZ12

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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px330 EZH2 - pDY052

Plasmid

#171107

Purpose

Expresses Cas9 and an sgRNA targeting EZH2

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pL2-G(TP)4TG

Plasmid

#137101

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G(TP)4TG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_485-938_H

Plasmid

#146457

Purpose

Bacterial Expression of NcQDE2_485-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_485-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM-60-NcQDE2_506-938_H

Plasmid

#146458

Purpose

Bacterial Expression of NcQDE2_506-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_506-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM-60-NcQDE2_517-938_H

Plasmid

#146459

Purpose

Bacterial Expression of NcQDE2_517-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_517-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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MvChR1_EYFP_pcDNA3.1

Plasmid

#159119

Purpose

This plasmid encodes MvChR1 rhodopsin domain

Depositor

John Spudich

Article

Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

Insert

MvChR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pIMAY

Plasmid

#68939

Purpose

E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

Depositor

Tim Foster

Article

Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

Type

Empty backbone

Use

Staphylococcal allelic exchange vector

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Article

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
px330 SUZ12 - pDY083

Plasmid

#171109

Purpose

Expresses Cas9 and an sgRNA targeting SUZ12

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
px330 EZH2 - pDY052

Plasmid

#171107

Purpose

Expresses Cas9 and an sgRNA targeting EZH2

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pL2-G(TP)4TG

Plasmid

#137101

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G(TP)4TG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM-60-NcQDE2_485-938_H

Plasmid

#146457

Purpose

Bacterial Expression of NcQDE2_485-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_485-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM-60-NcQDE2_506-938_H

Plasmid

#146458

Purpose

Bacterial Expression of NcQDE2_506-938

Depositor

Elisa Izaurralde

Article

Boland et al EMBO Rep. 2010 Jul;11(7):522-7. doi: 10.

Insert

NcQDE2_506-938

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge