We narrowed to 4,241 results for: Pet2;

1. pET28C-mCherry-MED1LCD

   Plasmid

   #204404

   Purpose

   E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   MED1(947-1581) (MED1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6his-WT gusA-pET28a+

   Plasmid

   #61156

   Purpose

   N -terminal His tagged wild-type GUS expression vector

   Depositor

   Ichiro Matsumura, Andrew Ellington

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   gusA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-miniE-DBCO

   Plasmid

   #181985

   Purpose

   Used to label proteins with the MiniR genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   MiniE-DBCO
   

   Use

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-HisYFP-Sp100

   Plasmid

   #53141

   Purpose

   E.coli expression of HisYFP tagged human Sp100 fragment

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   Sp100A fragment (SP100 Human)
   

   Use

   Tags

   His-YFP

   Expression

   Bacterial

   Mutation

   fragment (amino acids 71-480); deletion of L75

   Promoter

   T7

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-LgBiT-SpyCatcher

   Plasmid

   #223634

   Purpose

   Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   His6, LgBiT

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28C-mCherry-HNRNPA1LCD

   Plasmid

   #204405

   Purpose

   E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   HNRNPA1 (186-320) (HNRNPA1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28_6xHis-PARP1-CATdeltaHD

   Plasmid

   #174811

   Purpose

   Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CATdeltaHD (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residues 678-787 replaced by eight-residue linker…

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b-RBD-JA1

   Plasmid

   #167126

   Purpose

   Expresses SARS-CoV-2 RBD in bacterial system

   Depositor

   David Margulies

   Article

   Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

   Insert

   SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   residues (333 to 529 aa)

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28C-mCherry-DDX4LCD

   Plasmid

   #204408

   Purpose

   E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   DDX4(1-236) (DDX4 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-TEV-TBP6.9F34MF37MF77M

    Plasmid

    #168268

    Purpose

    Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    TBP6.9
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F34M, F37M and F77M

    Promoter

    T7 promotor

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-HIS-Tol2

    Plasmid

    #112486

    Purpose

    functional recombinant Tol2 protein from E.coli

    Depositor

    Stephen Ekker

    Article

    Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

    Insert

    Tol2 Transposase (tpase Synthetic)
    

    Use

    Tags

    HIS/6x

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-mH6-Cas12c1

    Plasmid

    #120872

    Purpose

    Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12c1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-mH6-Cas12c2

    Plasmid

    #120873

    Purpose

    Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12c2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28C-mCherry-53BP1LCD

    Plasmid

    #204407

    Purpose

    E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    53BP1(1208-1977) (TP53BP1 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28sumo-hRig-I DCARDS

    Plasmid

    #210828

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

    Insert

    hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28_mouse-pro-IL18

    Plasmid

    #183389

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (Il18 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21b-Tipin-His

    Plasmid

    #23122

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET28b(+)_rPD-L1nb

    Plasmid

    #226715

    Purpose

    Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag and Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET23b-Casp6-His

    Plasmid

    #11823

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 6 (CASP6 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-Ec.coaD (pESC106)

    Plasmid

    #50388

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Erick Strauss, Tadhg Begley

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    PPAT
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge