We narrowed to 7,293 results for: ef1a

1. pLV-EF1a-IRES-Puro PFKM

   Plasmid

   #201638

   Purpose

   Over-expression of untagged human PFKM

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   PFKM (PFKM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

   Plasmid

   #170220

   Purpose

   Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   T14A, Y15F

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EF1a-GFP-miRE_shRen-Puro

   Plasmid

   #135683

   Purpose

   Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-EF1aL-Cre-IRES-NeoR-W

   Plasmid

   #126700

   Purpose

   lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette

   Depositor

   Darrell Kotton

   Article

   Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

   Insert

   Cre-IRES
   

   Use

   Cre/Lox and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-Con/Fon-sRGECO

   Plasmid

   #137126

   Purpose

   Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-sRGECO
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E217D

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-Coff/Fon-GCaMP6M

   Plasmid

   #137121

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Coff/Fon-BFP

   Plasmid

   #137131

   Purpose

   Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1alpha-CSF2-218-PDGFR

   Plasmid

   #139058

   Purpose

   A lentivirus vector to integrate a surface membrane anchored human CSF2 gene

   Depositor

   Tao Liu

   Article

   Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

   Insert

   colony stimulating factor 2 (CSF2) (CSF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-EF1a-Neuromodulin-IRES-Puromycin

   Plasmid

   #134666

   Purpose

   Expresses EGFP-tagged plasma membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Neuromodulin (GAP43 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-Con/Fon-BFP

    Plasmid

    #137129

    Purpose

    Intersectional viral expression of BFP in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_EF1a-->Gss52SR4-LaM4-P2A-NLSIFP

    Plasmid

    #162229

    Purpose

    Lentiviral vector for the expression of Gss52SR4-LaM4-P2A-NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaG17 GFP nanobody SynNotch tTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-DIO-VMAT2-WPRE-pA

    Plasmid

    #39339

    Depositor

    Bernardo Sabatini

    Article

    Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV; R

    Tags

    Expression

    Mutation

    Myc tag inserted in the first lumenal loop betwee…

    Promoter

    Available since

    Oct. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-Coff/Fon-GCaMP6F

    Plasmid

    #137124

    Purpose

    Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-GCaMP6F
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. P-Ef1a-EGFP-T2A-RPL22-1xFlag

    Plasmid

    #170323

    Purpose

    Expresses FLAG and EGFP tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-loxP-GRAB_DA2m-loxP

    Plasmid

    #189751

    Purpose

    Excludes sensor expression in Cre-positive neurons

    Depositor

    Yulong Li

    Article

    Dai et al Cell Rep. 2022 Aug 23;40(8):111246. doi:

    Insert

    Dopamine sensor GRAB_DA2m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-DIO-FLPo-Myc

    Plasmid

    #124641

    Purpose

    AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    FLPo
    

    Use

    AAV and Cre/Lox

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-EF1A-CD19.8H.8TM.BB.28.3z

    Plasmid

    #200673

    Purpose

    Modular CD8hinge-CD8transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-EF1alpha-IRES-EGFP-HA-hNPHP1

    Plasmid

    #122874

    Purpose

    Expresses HA-tagged human NPHP1 in mammalian cells

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Insert

    human NPHP1 (NPHP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

    Plasmid

    #159756

    Purpose

    PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid

    #105715

    Purpose

    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

    Depositor

    Rylan Larsen

    Has Service

    AAV5

    Insert

    synaptophysin-GCaMP6s
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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