We narrowed to 9,864 results for: plasmids 101

1. P1m

   Plasmid

   #120934

   Purpose

   MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.

   Depositor

   Richard Murray

   Insert

   J23101
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVTRE3_Clover

   Plasmid

   #135179

   Purpose

   AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Inserts

   Clover
   TRE3G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   a variant of GFP

   Promoter

   TRE3G

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1_Cre

   Plasmid

   #201198

   Purpose

   AAV vector for cre expression under EF1 promoter

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Inserts

   cre
   EF1alpha promoter
   

   Use

   AAV

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   nuclear targeting cre

   Promoter

   N/A and hEF1alpha promoter

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

   Plasmid

   #74102

   Purpose

   plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Xenopus laevis beta-tubulin gene promoter region and 5'UTR
   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS2326

   Plasmid

   #169628

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2317

   Plasmid

   #169624

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-Copper ATPase 1 [L60/4R-rat IgG2a] - Chimeric

   Plasmid

   #231823

   Purpose

   Mammalian expression plasmid of anti-Copper ATPase 1 (Menke's disease protein) (Human) rat IgG2a R-mab. Derived from hybridoma L60/4.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-Copper ATPase 1 (Menke's disease protein) (Homo sapiens) recombinant mouse monoclonal antibody (ATP7A Chimera: M. musculus (mouse)/R. norvegicus (rat))
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-Kir2.3 K+ channel [N25/35R-rat IgG2a] - Chimeric

   Plasmid

   #231848

   Purpose

   Mammalian expression plasmid of anti-Kir2.3 K+ channel (Human) rat IgG2a R-mab. Derived from hybridoma N25/35.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-Kir2.3 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNJ4 Chimera: M. musculus (mouse)/R. norvegicus (rat))
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. FHRE-Luc

   Plasmid

   #1789

   Purpose

   Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

   Depositor

   Michael Greenberg

   Article

   Brunet et al Cell 1999 Mar 19;96(6):857-68.

   Insert

   FHRE (FOXO3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   3 copies of FRE

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL90-Nat-2xHO

    Plasmid

    #139069

    Purpose

    CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Insert

    Two guide RNAs targeting the HO endonuclease
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-DEST42-ARL4-V5-His6

    Plasmid

    #67419

    Purpose

    Expression of hArl4 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4 (ARL4A Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    bp 105 A to T; silent mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-hSyn-DIO-Gluc-NHP.RMA-IRES-EGFP

    Plasmid

    #227887

    Purpose

    Expresses Gluc-NHP.RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-NHP.RMA and EGFP for monitoring Cre-expressing neuronal populations.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al bioRxiv 2025.06.01.657212; doi: 10.1101/

    Inserts

    Gaussia luciferase fused to macaque Fc
    IRES-EGFP
    

    Use

    AAV, Cre/Lox, and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. psicheck2-B4GALT3

    Plasmid

    #203601

    Purpose

    Reporter plasmid carrying B4GALT3 3'UTR

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    beta-1,4-galactosyltransferase 3 (B4GALT3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    nt 1800-2330 3'UTR

    Promoter

    SV40

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid

    #169659

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST40-ARL14-V5-His6

    Plasmid

    #67448

    Purpose

    Mammalian expression of hArl14 with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL14 (ARL14 Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    bp 172 C to T; aa L58F

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET-DEST42-ARL14-V5-His6

    Plasmid

    #67427

    Purpose

    Expression of hArl14 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL14 (ARL14 Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    bp 172 C to T; aa L58F

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET-DEST42-ARFRP1-V5-His6

    Plasmid

    #67429

    Purpose

    Expression of hArfrp with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pPSU1

    Plasmid

    #89439

    Purpose

    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders

    Depositor

    Song Tan

    Article

    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

    Inserts

    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Anti-Cav1.2/1.3 Ca2+ channel [L57/46R]

    Plasmid

    #177485

    Purpose

    Mammalian Expression Plasmid of anti-Cav1.2/1.3 Ca2+ channel (Rabbit). Derived from hybridoma L57/46.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Cav1.2/1.3 Ca2+ channel (Oryctolagus cuniculus) recombinant mouse monoclonal antibody (CACNA1C, CACNA1D Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLD-CAG-DYSF-CFL

    Plasmid

    #131457

    Purpose

    Encodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    DYSF and Follistatin (DYSF, FST Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    R15C (in DYSF) and Q2579H (in CFL)

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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