We narrowed to 6,524 results for: poly

1. GA46

   Plasmid

   #196614

   Purpose

   Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
   

   Use

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUGP1.1

   Plasmid

   #196615

   Purpose

   Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
   

   Use

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

   Plasmid

   #176236

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176237

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

   Plasmid

   #176238

   Purpose

   Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Insert

   spCas9 and polymerase exo domain fusion (CLCN5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1delZn1-L713F

   Plasmid

   #173937

   Purpose

   Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn1-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-96; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-PARP1delZn3-L713F

   Plasmid

   #173934

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-TSV (linearized in VP2)

   Plasmid

   #70032

   Purpose

   pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region

   Depositor

   Mariet Feltkamp

   Article

   van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

   Insert

   TSV full genome, linearized in VP2 gene
   

   Use

   Cloning vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-EF1-FHC-POLQ-DY2230AA

   Plasmid

   #64878

   Purpose

   lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   D2330A,Y2331A, in the DNA polymerase domain (POL)…

   Promoter

   EF1alpha

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pwM

    Plasmid

    #22515

    Depositor

    Christopher Buck

    Article

    Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

    Insert

    MCPyV VP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon modified gene

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. ph2m

    Plasmid

    #22518

    Depositor

    Christopher Buck

    Article

    Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

    Insert

    MCPyV VP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon modified

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-pIgR cDNA

    Plasmid

    #12109

    Depositor

    Pamela Bjorkman

    Article

    Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

    Insert

    human polymeric immunoglobulin receptor (PIGR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pET20b-pIgR D1-H6

    Plasmid

    #12530

    Depositor

    Pamela Bjorkman

    Article

    Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

    Insert

    human polymeric immunoglobulin receptor (PIGR Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Lys 1-Val 109

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

15. AP-p003

    Plasmid

    #31494

    Depositor

    David Wang

    Article

    Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

    Insert

    Half genome of WU polyomavirus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pAcGP67A-pIgR D1-H6

    Plasmid

    #12531

    Depositor

    Pamela Bjorkman

    Article

    Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

    Insert

    human polymeric immunoglobulin receptor (PIGR Human)
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Lys 1 - Val 109

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

17. AP-p002

    Plasmid

    #31493

    Depositor

    David Wang

    Article

    Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

    Insert

    Half genome of WU polyomavirus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

18. GGE114

    Plasmid

    #120731

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly. It already has some of the parts needed for transformation in Y. lipolytica

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Type

    Empty backbone

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMpGE010-gR082

    Plasmid

    #188553

    Purpose

    Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

    Depositor

    Yutaka Kodama

    Article

    Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

    Insert

    gR082
    

    Use

    CRISPR; Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GGE367

    Plasmid

    #120736

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Antibiotic resistance marker for selection after transformation in Y. lipolytica (Marker_hph)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    Marker_hph
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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