We narrowed to 17,101 results for: Por

1. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

   Plasmid

   #112669

   Purpose

   Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

   Depositor

   Catherine Verfaillie

   Article

   Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

   Insert

   tdT
   

   Use

   AAV; Donor plasmid for recombinase-mediated casse…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

   Plasmid

   #114428

   Purpose

   Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GCaMP6f
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #223321

   Purpose

   It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Inserts

   SpCas9 codon optimized for human (cas9 Human, Synthetic)
   chloramphenicol resistance protein
   CcdB
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

   Plasmid

   #229770

   Purpose

   Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
   

   Use

   Luciferase

   Tags

   LgBiT

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

   Plasmid

   #229769

   Purpose

   Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
   

   Use

   Luciferase

   Tags

   SmBiT

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-CRY2oligo-mcherry-ANXA11(D40G)

   Plasmid

   #164204

   Purpose

   Expresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Tags

   CRY2oligo-mcherry

   Expression

   Mammalian

   Mutation

   D40G

   Promoter

   CMV

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFN33K LgBiT TK-neo Human collagen type IV alpha 5 chain (COL4A5)

   Plasmid

   #229768

   Purpose

   Human Col4A5 tagged at N-terminal with LgBiT to be used with N-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

   Depositor

   Hirofumi Kai

   Article

   Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

   Insert

   Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)
   

   Use

   Luciferase

   Tags

   LgBiT

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

   Plasmid

   #231549

   Purpose

   Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   msfGFP (intramolecular)

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVtrunc msfGFPΔC10-beta-actin

   Plasmid

   #231548

   Purpose

   Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid

    #229767

    Purpose

    Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    

    Use

    Luciferase

    Tags

    SmBiT

    Expression

    Mutation

    See Depositor Comments

    Promoter

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

    Plasmid

    #203797

    Purpose

    Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Article

    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

    Inserts

    PIK3CA (PIK3CA Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221-SLC32A1_STOP

    Plasmid

    #161278

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC32A1 (SLC32A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pTRE3G-GSX2-IRES2-EBF1

    Plasmid

    #96966

    Purpose

    Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)
    

    Use

    Tags

    FLAG (on EBF1)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-FLVCR1_STOP

    Plasmid

    #161058

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    FLVCR1 (FLVCR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLenti_CMVTRE3G_LYSET-Isoform2_Flag

    Plasmid

    #191999

    Purpose

    Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-Isoform2 (LYSET Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMVTRE3G

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_CMVTRE3G_LYSET-Isoform1_untagged-Puro

    Plasmid

    #192001

    Purpose

    Lentiviral vector to generate LYSET(TMEM251)-isoform1 stable expressing cell line under CMVTRE3G promoter

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-Isoform1 (LYSET Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVTRE3G

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GAP50-COMP-blac-flag-his

    Plasmid

    #110956

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    secreted acid phosphatase (GAP50) (PF3D7_0918000 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAEBL-COMP-blac-flag-his

    Plasmid

    #110963

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    merozoite adhesive erythrocytic binding protein (MAEBL) (PF3D7_1147800.1 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS426-HsFECH

    Plasmid

    #174524

    Purpose

    Expresses human ferrochelatase (FECH) in yeast

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    FECH with yeast HEM15 promoter and terminator (FECH Human, Synthetic, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Lacks original mitochondrial targeting sequence (…

    Promoter

    HEM15 (yeast, 257 bp)

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald

    Plasmid

    #203798

    Purpose

    Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Article

    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

    Inserts

    PIK3CA (PIK3CA Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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