165,839 results

1. pHygG6

   Plasmid

   #200219

   Purpose

   hygR selection GFP marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF3352

   Plasmid

   #144828

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HESX1 (HESX1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-SIRPB1-COMP5AP-AviTag-9xHis

   Plasmid

   #157344

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SIRPB1 (SIRPB1 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF0181

   Plasmid

   #143390

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ESX1 (ESX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. VAMP8

   Plasmid

   #186689

   Purpose

   Expresses VAMP8 in E. Coli

   Depositor

   Axel Brunger

   Article

   Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

   Insert

   VAMP8
   

   Use

   Tags

   3xFLAG, GST, TEV, and Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.U6gT28.4.hSyn.H2B.RFP

   Plasmid

   #170387

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B RFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 20 pCOLI_A_N-His_C_STREP

   Plasmid

   #160162

   Purpose

   N-term 6xHis 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   2xStrepII and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE6g

   Plasmid

   #207857

   Purpose

   Mammalian expression of PE6g prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6g
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

   Promoter

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLAG-CMV-hErk1

   Plasmid

   #49328

   Purpose

   Expresses FLAG-tagged human Erk1

   Depositor

   Melanie Cobb

   Insert

   Erk1 (MAPK3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-HDAC6-FLAG

    Plasmid

    #30482

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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