We narrowed to 10,808 results for: T7

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pEE008

Plasmid

#176765

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

104

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE009

Plasmid

#176766

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

201

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE010

Plasmid

#176767

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

202

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED(216-242)+CBPTAZ1(340-439)

Plasmid

#189961

Purpose

Coexpression of CBP TAZ1 and CITED in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-242) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)

Plasmid

#189963

Purpose

Coexpression of CBP TAZ1 and CITED2 in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-269) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Mutation

L243A, E245A, L246A

Promoter

t7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)

Plasmid

#189962

Purpose

Expression of CBP TAZ1 and CITED2 in E coli

Depositor

Peter Wright

Article

Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

Inserts

CITED2 (216-246) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Expression

Bacterial

Promoter

t7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP-IST1 N-terminal domain

Plasmid

#184805

Purpose

Bacterial expression of IST1 N-terminal domain

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

IST1 (IST1 Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

aa 1-187 only

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET303C-hSOD1 A4V/C6S

Plasmid

#139667

Purpose

Expression plasmids containing human A4V/C6S SOD1

Depositor

Svetlana Antonyuk

Article

Chantadul et al Commun Biol. 2020 Mar 5;3(1):97. doi: 10

Insert

Superoxide dismutase-1 (SOD1 Human)

Expression

Bacterial

Mutation

change alanine 4 to valine, change cysteine 6 to …

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP-CHMP1B, S2C

Plasmid

#184803

Purpose

Bacterial expression of CHMP1B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

CHMP1B (CHMP1B Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-cPHx1

Plasmid

#183674

Purpose

PtdIns(3,4)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 169-329

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET-SU(Z)12(624-900)-H6

Plasmid

#183772

Purpose

Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

Insert

SU(Z)12 residues 624-900 (Su(z)12 Fly)

Tags

His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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iDianiSnFR

Plasmid

#177741

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iDianiSnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CEA-N domain residues 1-132 (CEACAM5)-pET30b

Plasmid

#174693

Purpose

Plasmid expressing CEA-N domain

Depositor

Jean Gariepy

Article

Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

Insert

CEA-N Domain

Tags

TEV Cleavage Site and hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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iCytSnFR

Plasmid

#177738

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCytSnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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iCyt_BrEt_SnFR

Plasmid

#177740

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCyt_BrEt_SnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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iCyt_F_SnFR

Plasmid

#177739

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCyt_F_SnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

Plasmid

#175051

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)

Expression

Bacterial

Mutation

changed Thr76 to Asp, phosphomimetic mutation

Promoter

T7 promoter

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

Plasmid

#175050

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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