167,948 results

1. SPHK2 gRNA (BRDN0001145867)

   Plasmid

   #77094

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXJ-Myc-GATA4

   Plasmid

   #232647

   Purpose

   transient expresses GATA4 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

   Insert

   GATA4 (GATA4 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. CIBN-CAAX

   Plasmid

   #79574

   Purpose

   expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

   Depositor

   Olof Idevall-Hagren, Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Tags

   C-terminal CAAX-box

   Expression

   Mammalian

   Mutation

   K93A, R94A, K106A, K107A (please see depositor co…

   Promoter

   CMV

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-Ftractin-mCherry

   Plasmid

   #85131

   Purpose

   lentiviral expression of an F-actin live-cell marker (mCherry)

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPdX

   Plasmid

   #85084

   Purpose

   Expresses putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Cysteine 73 to Serine and Cysteine 85 to …

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. ALKBH5-dCas9

   Plasmid

   #134783

   Purpose

   Expression fusion protein ALKBH5-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   ALKBH5 and inactive dCas9
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CCR5-Tango

   Plasmid

   #66242

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CCR5 (CCR5 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. PL452-hGH-polyA-PGK-neoR

   Plasmid

   #191681

   Purpose

   Donor plasmid backbone PGK-neoR

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Type

   Empty backbone

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_Lifeact_HaloTag9

   Plasmid

   #175545

   Purpose

   Expression of HaloTag9 at the actin filaments of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-HA-Ubiquitin-K29

    Plasmid

    #22903

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K29 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-Cytb5

    Plasmid

    #182579

    Purpose

    Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Cytb5
    

    Tags

    Cytb5

    Expression

    Mammalian

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEYFP-C1-Drp1

    Plasmid

    #45160

    Depositor

    Richard Youle

    Article

    Frank et al Dev Cell. 2001 Oct;1(4):515-25.

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. bMS.346

    Bacterial Strain

    #220588

    Purpose

    Increase editing efficiency of retron and oligo recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Bacterial Resistance

    None

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pNF02-mChartreuse

    Plasmid

    #219397

    Purpose

    Constitutive expression of mChartreuse in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mChartreuse
    

    Expression

    Bacterial

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-EGFP (AAV1 trial size)

    Viral Prep

    #50465-AAV1.T

    Purpose

    Ready-to-use AAV1 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pBS-SKII-3XHA-HALO-NAT

    Plasmid

    #188931

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p102_gRNA_Sa_mClover

    Plasmid

    #213778

    Purpose

    Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sa gRNA
    

    Use

    CRISPR and Lentiviral

    Promoter

    mU6

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p103_gRNA_Sp_mClover

    Plasmid

    #213779

    Purpose

    Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sp gRNA
    

    Use

    CRISPR and Lentiviral

    Promoter

    mU6

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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