We narrowed to 23,555 results for: ESC
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Plasmid#45579PurposeExpresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani K227M
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZJOM44
Plasmid#133653PurposeIntegration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAST_NSlmb-vhhGFP4
Plasmid#35575DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
ExpressionInsectAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Sec61β
Plasmid#174466PurposeExpress EGFP with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertSirius with endoplasmic reticulum-targeting sequence
TagsSec61 subunit betaExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-Ef1a-TVA-2A-oG
Plasmid#172359PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neuronsDepositorInsertTVA + B19-oG
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB4542
Plasmid#215261PurposeModule for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19DepositorInsertCBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4401
Plasmid#215252PurposeModule for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertSF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Nup35-mut
Plasmid#87341PurposeTo express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP35 (NUP35 Human)
TagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CALPAIN-7-mCherry
Plasmid#180652PurposeLentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.DepositorInsertCALPAIN-7
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GCACCCAUACCUUUACAUUAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
BAS286 C terminal SF-GFP hygBR
Plasmid#74081PurposeS.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistancDepositorInsertsuper-folder GFP
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1_rpsM_mCerulean3
Plasmid#124904PurposerpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)DepositorInsertrpsM promoter and mCerulean3
ExpressionBacterialAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTD-C_sfYFPTwinStrep
Plasmid#45943Purposeexpresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion proteinDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
TagsTwin-Strep-tagExpressionBacterialPromoterlacIq/PtrcAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFX-mKate2-Rab11
Plasmid#174463PurposeExpress mKate2 with recycling endosome-targeting sequence in mammalian cellsDepositorInsertmKate2 with recycling endosome-targeting sequence
Tagssmall GTPase (Rab11)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_2
Plasmid#78206PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter bindingDepositorInsertmatB (His6 C106A S170A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A S170A R286C Q457C K488APromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
ExpressionBacterialAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AA294
Plasmid#215968PurposeFragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoterDepositorHas ServiceCloning Grade DNAInsertU1a_v1
UseFragmentAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA332
Plasmid#216053PurposeFragmid fragment: (C' terminus) 3x NLS sequencesDepositorHas ServiceCloning Grade DNAInsertSV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (pGEX2T(TEV))
Plasmid#80630PurposeBacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197DepositorAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZJOM22
Plasmid#133629PurposeIntegration of EMC1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM70
Plasmid#133658PurposeIntegration of ERG6-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only