We narrowed to 24,327 results for: Sis

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pBXPHM3-NB-D6

Plasmid

#167989

Purpose

expression of a nanobody against rat PepT2 as a n terminal MBP fusion

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

NanobodyD6

Tags

His-MBP

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pIALD2E

Plasmid

#185866

Purpose

Knocking out ALD6

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

ALD(-125, 40)- ALD6(1054, 1749)

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST tol2 UbB polyA

Plasmid

#188701

Purpose

Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0033

Plasmid

#185624

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-2

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0032

Plasmid

#185623

Purpose

Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

NbU6-1

Use

CRISPR and Synthetic Biology

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK028

Plasmid

#180826

Purpose

T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

SwGdmA (SWIT_RS16490 Synthetic, Sphingomonas wittichii RW1)

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK037

Plasmid

#180829

Purpose

T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

CnGdmB (CNE_RS35790 Cupriavidus necator N-1)

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK066

Plasmid

#180830

Purpose

T7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

NaGdmA

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0170

Plasmid

#172813

Purpose

Level 1 module containing nCFP_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-SP_SV40-CFP-3U+TERNOS

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0080

Plasmid

#172786

Purpose

Level 0 module containing NT2_nVenus

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

3X FLAG CtmVENUS 210-238 RSIAT pICH41258 (SP NT2)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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GST_LIM1224

Plasmid

#182041

Purpose

For expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM1224 (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

LIM domains 1, 2, 2 and 4

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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GST_LIM1334

Plasmid

#182040

Purpose

For expression of human paxillin LIM domains 1, 3, 3 and 4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM1334 (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

LIM domains 1, 3, 3 and 4

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/TARG1

Plasmid

#172580

Purpose

For generation of FlpIn lines with untagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-TARG1

Plasmid

#172574

Purpose

For gateway cloning of N-terminal tagged TARG1 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Gateway cloning

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmPAN3_349-790-P652G_T

Plasmid

#147570

Purpose

Bacterial Expression of DmPAN3_349-790-P652G

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3_349-790-P652G (CG11486 Fly)

Expression

Bacterial

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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Pb mD3S4-dN

Plasmid

#180252

Purpose

P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

Depositor

Timothy Springer

Article

Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

Insert

D3 of P. berghei HAP2 with N-glycosylation sites mutated

Tags

6His

Expression

Mammalian

Mutation

N516T, S533N and N539Q

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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IBAR-Cry2PHR-WH2

Plasmid

#176605

Purpose

Cry2PHR with N-terminal IBAR domain and C-terminal WH2 domain

Depositor

Robert Hughes

Article

Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

Insert

IBAR-Cry2PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-DFDF-KFKF_C

Plasmid

#146036

Purpose

Insect Expression of DmEDC3-DFDF-KFKF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-DFDF-KFKF (Edc3 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-FDF-ADA_C

Plasmid

#146037

Purpose

Insect Expression of DmEDC3-FDF-ADA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-FDF-ADA (Edc3 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-pntAB

Plasmid

#158609

Purpose

Low phosphate induction of pntAB

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)

Expression

Bacterial

Promoter

Insulated ugpBp from E. coli

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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