We narrowed to 1,399 results for: pBAD
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Plasmid#149387PurposeP. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation6bp deleted downstream of the IHFprox sitePromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_-5D
Plasmid#149388PurposeP. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation5bp deleted downstream of the IHFprox sitePromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+5D
Plasmid#149390PurposeP. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation5bp inserted downstream of the IHFprox sitePromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D
Plasmid#149391PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation10bp inserted downstream of the IHFprox sitePromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Opt
Plasmid#149395PurposeP. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationIHFprox site is optimized at key positions needed…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL1514
Plasmid#155147PurposeExpresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaDDepositorInsertmlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1965
Plasmid#155152PurposeExpresses MlaF(1-246)-(6 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1966
Plasmid#155153PurposeExpresses MlaF(1-246)-(10 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1967
Plasmid#155154PurposeExpresses MlaF(1-246)-(14 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0.093
Plasmid#119574PurposeLevel 0 part. PromoterDepositorInsertPBAD
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2m
Plasmid#105066PurposeA yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cellsDepositorInsertY-GECO2m
Tags6x His tagExpressionBacterialPromoterpBADAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVH004
Plasmid#80401PurposeContains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.DepositorUseSynthetic BiologyTagsC-terminal LZx domainExpressionBacterialAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH015
Plasmid#80398PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blotsDepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHYBAD94
Plasmid#42305DepositorInsertNpuDnaEC6
TagsGB1-H6ExpressionBacterialPromoteraraBADAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2s
Plasmid#105067PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2s
Tags6x His tagExpressionBacterialPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.1s
Plasmid#105068PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.1s
Tags6x His tagExpressionBacterialPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
ExpressionBacterialPromoterglnS and pBADAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
TagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialPromoterpDABAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-8
Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterP(rhaB)Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRExpressionBacterialPromoterpBADAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only