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pBbdCas9S_P0826S-sgRNAflaB

Plasmid

#149647

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, P0826S

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAftsI

Plasmid

#149649

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAftsI

Plasmid

#149650

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, P0526

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9G(-10TC)_arr2

Plasmid

#149585

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9B(-10TC)_arr2

Plasmid

#149586

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9B_arr2

Plasmid

#149572

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9H_arr2

Plasmid

#149573

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9S(RBSmut)_arr2

Plasmid

#149575

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9B(RBSmut)_arr2

Plasmid

#149579

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9H(RBSmut)_arr2

Plasmid

#149580

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2H_Psyn-sgRNAftsI

Plasmid

#149563

Purpose

ftsI gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2H_Psyn-sgRNAmreB

Plasmid

#149567

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E-161K enhancer

Plasmid

#138365

Purpose

Gateway compatible 5' entry clone contains human modified 161k enhancer sequence.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

161k human enhancer

Use

Gateway compatible

Tags

Expression

Mutation

9 A bases deletion, that have no impact on the en…

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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CALCOCO1 L140A-mycDDK

Plasmid

#137767

Purpose

CALCOCO1 CLIR mutant

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 L140A

Use

Tags

mycDDK

Expression

Mammalian

Mutation

L140A

Promoter

CMV

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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CALCOCO1 R12H-mycDDK

Plasmid

#137770

Purpose

CALCOCO1 R12H mutant

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 R12H

Use

Tags

mycDDK

Expression

Mammalian

Mutation

R12H

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX2AHK

Plasmid

#118826

Purpose

Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSox2AHK (Sox2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

AHK: E46A, I53H

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEX Tom20 mVenus BoPD

Plasmid

#114466

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoPD

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoPD

Use

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTICRISPR sgNFS1

Plasmid

#102979

Purpose

Cutting NFS1 genomic locus

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

sgNFS1 (NFS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G11-R0

Plasmid

#101805

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

Sven van Teeffelen, David Bikard

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G0-R11

Plasmid

#101808

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

Sven van Teeffelen, David Bikard

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G0-R18

Plasmid

#101813

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

Sven van Teeffelen, David Bikard

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G14-R0

Plasmid

#101806

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

Sven van Teeffelen, David Bikard

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G10-R0

Plasmid

#101804

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

Sven van Teeffelen, David Bikard

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413 Prp5 AAAA

Plasmid

#87600

Purpose

Encodes Prp5 AAAA for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

D136A, P137A, L138A, D139A

Promoter

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413 Prp5 TAG

Plasmid

#87603

Purpose

Encodes Prp5 SAT motif to TAG for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

S446T, T448G

Promoter

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 K335R

Plasmid

#87299

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335R

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335R

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBKT7-CLF1

Plasmid

#87548

Purpose

Encodes Y2H Gal4 DNA binding domain for Clf1

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

CLF1 (CLF1 Budding Yeast)

Use

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 D450G

Plasmid

#87303

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 D450G

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

D450G

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 P369K

Plasmid

#87302

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 P369K

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

P369K

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 H331Q

Plasmid

#87296

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 H331Q

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

H331Q

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 H331D

Plasmid

#87295

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 H331D

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

H331D

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 N295D

Plasmid

#87294

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 N295D

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

N295D

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 R294L

Plasmid

#87293

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 R294L

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

R294L

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414 Hsh155 N295D/D450G

Plasmid

#87287

Purpose

Encodes Hsh155 N295D D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

N295D & D450G

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414 Hsh155 R294L/H331D

Plasmid

#87282

Purpose

Encodes Hsh155 R294L H331D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

R294L & H331D

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414 Hsh155 N295D/H331D

Plasmid

#87285

Purpose

Encodes Hsh155 N295D H331D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

N295D & H331D

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 P369E

Plasmid

#87301

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 P369E

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

P369E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 K335T

Plasmid

#87300

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335T

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335T

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 K335N

Plasmid

#87298

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335N

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335N

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 K335E

Plasmid

#87297

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335E

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2041 - 2080 of 22214 results

Results per page:

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