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pBABEpuro-Flag-ZO-2

Plasmid

#27420

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pSFFV_mNG3A(1-10)

Plasmid

#157992

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

W148L, M150V, K153M, N194K, F204L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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SERK2 P5_pECIA14

Plasmid

#114768

Purpose

Prey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 P5_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34210 (SERK2 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK2 P5_pECIA2

Plasmid

#114968

Purpose

Bait vector SERK2 P5_pECIA2 should be used with prey vector SERK2 P5_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34210 (SERK2 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-sgRNA-Sirius-8XMS2

Plasmid

#121942

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*11:01-BirA

Plasmid

#235135

Purpose

E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*11:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

G120C

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-hKlf4

Plasmid

#20074

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hKlf4 (KLF4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pJRH-1363 LV EF1a-CD19 IRES-EGFP

Plasmid

#201919

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD19 (CD19 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-PuroR (ANV)

Plasmid

#71684

Purpose

Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

CBh

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

Plasmid

#44027

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P405A, P530V, N851A

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2-MT mouse Axin (Axin MTFu1)

Plasmid

#21287

Depositor

Frank Costantini

Article

Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Insert

Axin (Axin1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-11_humanized-mutant

Plasmid

#214316

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Use

Retroviral

Tags

Myc

Mutation

KLS288YKT_KASIHS348TPRAKA

Promoter

MESV

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-11_humanized-mutant

Plasmid

#214315

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Tags

His-TEV

Expression

Bacterial

Mutation

N152K_KLS288YKT_KASIHS348TPRAKA

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pChREBP

Plasmid

#39235

Depositor

Isabelle Leclerc

Article

da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Ep

Insert

ChREBP (Mlxipl Mouse)

Tags

cMyc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
DHFR-SpCas9-DHFR

Plasmid

#85447

Purpose

Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-SpCas9-DHFR

Use

AAV

Tags

Destabilized domain (C-terminus version) of E.col…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1-1482 aa)

Plasmid

#21745

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1-1482) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1-1482

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
Flag-M4-AR

Plasmid

#171240

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor (AR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p026 pCS2-EnR

Plasmid

#11028

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

engrailed (en Fly)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Engrailed repressor domain (bases encoding amino …

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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