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iSPI_pSer_Subpool#3

Pooled Library

#188528

Purpose

Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
iSPI_pSer_Subpool#4

Pooled Library

#188529

Purpose

Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
iSPI_pSer_Subpool#6

Pooled Library

#188531

Purpose

Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
iSPI_pSer_Subpool#7

Pooled Library

#188532

Purpose

Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
iSPI_pSer_Subpool#8

Pooled Library

#188533

Purpose

Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
iSPI_pSer_Subpool#9

Pooled Library

#188534

Purpose

Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
iSPI_pSer_Subpool#10

Pooled Library

#188535

Purpose

Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
iSPI_pSer_Full_library

Pooled Library

#188536

Purpose

Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
145-pGL3ctrl-3' UTR

Plasmid

#28175

Depositor

Michael Kastan

Article

Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

Insert

Luciferase flanked by tp53 UTRs (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Note that insert length is based on sum of the 2 …

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pVRa20_992

Plasmid

#49673

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF20_992

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pTRE-FDXR wt

Plasmid

#16610

Depositor

Bert Vogelstein

Article

Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

Insert

FDXR (FDXR Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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p5E_GGWDest+

Plasmid

#49319

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p5E_GGWDest-

Plasmid

#49318

Purpose

Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_XcmI-LacZ

Plasmid

#49296

Purpose

Golden Gate entry vector at position 1, no 5' ATG, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_Linker

Plasmid

#49281

Purpose

Golden Gate entry vector at position 1, small linker insert, no 5' ATG

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti TurboRFP BlastR

Plasmid

#102343

Purpose

Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

TurboRFP

Use

Lentiviral

Tags

N/A

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-PYL1-sfGFP-PML

Plasmid

#167011

Purpose

expression of PYL1-SfGFP-PML, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

PML (PML Human)

Use

Lentiviral

Tags

GFP-PML

Expression

Mammalian

Available Since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX

Plasmid

#111516

Purpose

Expresses E. coli ClpX with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

Plasmid

#169449

Purpose

expression of TRF1-mcherrry and sgRNA targeting telomeres

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

mCherry-TRF1

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mRuby2 BlastR

Plasmid

#84384

Purpose

Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

Lifeact

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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