Addgene

We narrowed to 1,214 results for: 5-Oct

Showing: 1021 - 1040 of 1214 results

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pAAV-gfaABC1D-HA-deLACCO1-NGR

Plasmid

#208099

Purpose

Control biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet, Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK-hACE2

Plasmid

#161612

Purpose

Expression of human ACE2 in mammalian cells

Depositor

Brad Rosenberg

Article

Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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U6-sgfiller-eCas9-T2A-BlastR

Plasmid

#172437

Purpose

Expresses eCas9, BlastR and sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

eCas9
BlastR

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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LV-PGK-Cre-EFS-mScarletSIIN

Plasmid

#172434

Purpose

Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry

Plasmid

#208835

Purpose

Knockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry

Plasmid

#208836

Purpose

Knockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry

Plasmid

#208837

Purpose

Knockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Hyg-Snrnp40-skywarp-CRISPR-resistant

Plasmid

#134252

Purpose

Lentivector encoding CRISPR-resistant skywarp form of Snrnp40 (missing exon 5)

Depositor

Bruce Beutler

Article

Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

Insert

Snrp40 (Snrnp40 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

deleted amino acids 179-219, and mutated coding s…

Promoter

EF1a

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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Cer(1-158)-beta-1 in pcDNAI/Amp

Plasmid

#55707

Purpose

An amino-terminal fragment of mCerulean was fused to Gbeta1. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal s produced.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

mCer(1-158)-beta 1 (GNB1 Aequorea victoria, Human)

Use

Tags

Cer(1-158) was fused to the amino terminus of Gbe…

Expression

Mammalian

Mutation

GBeta-1 was amplified via PCR, which removed an i…

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-stGtACR2-FusionRed

Plasmid

#217975

Purpose

AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

Depositor

Edward Callaway

Article

Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

Insert

stGtACR2-FusionRed

Use

AAV

Tags

FusionRed

Expression

Mutation

Promoter

EF1a

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEB-C5

Plasmid

#28213

Purpose

5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Jan 18. ():.

Insert

Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2011

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-eGFP

Plasmid

#164519

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-NGFR

Plasmid

#164520

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28c S2

Plasmid

#171920

Purpose

Express RNA-based (p)ppGpp sensor

Depositor

Mingxu You

Article

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Insert

RNA-based (p)ppGpp sensor

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pETDuet S2

Plasmid

#171921

Purpose

Express RNA-based (p)ppGpp sensor and a NirFP

Depositor

Mingxu You

Article

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Inserts

RNA-based (p)ppGpp sensor
NirFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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L13-Arl13bGFP

Plasmid

#40879

Purpose

3rd generation transfer vector

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST47-Arl13b12

Plasmid

#40872

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pSUP-(2xCouRS-tRNAopt)

Plasmid

#197930

Purpose

Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

Depositor

Kenneth Johnson

Article

Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

Inserts

CouRS
tRNA opt

Use

Tags

Expression

Bacterial

Mutation

Promoter

glnS' and lambda(ts) and proK

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLX301-ZIM3-KRAB-PYL1

Plasmid

#154761

Purpose

Expresses ZIM3 KRAB domain fused to PYL1

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

CMV

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCIG3 N

Plasmid

#132941

Purpose

MLV packaging plasmid for N-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

N-tropic MLV gag-pol

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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