We narrowed to 2,568 results for: CCS
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Plasmid#172846PurposeCRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
gCH29 (crCD81-1)
Plasmid#217344PurposecrRNA targeting CD81DepositorInsertcrCD81-1 (CD81 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer
Plasmid#90013PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-DicerDepositorInsertsCBirA*-Ago2
NBirA*-Dicer
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shSMAD4 #1
Plasmid#83089PurposeLentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-TRIM28deltaLinker
Plasmid#124958PurposeMammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domainsDepositorInserthTRIM28 (TRIM28 Human)
UseTagsHAExpressionMammalianMutationDeletion of linker region between CC domain and P…PromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-PLAA
Plasmid#85669PurposeExpression of PLAA with N-terminal GFP tagDepositorInsertPLAA (PLAA Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3HA-hMIB2
Plasmid#33312DepositorInsertMIB2 (MIB2 Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
NSK021 CMV-TO-p53R248Q (FLP-IN)
Plasmid#191167PurposeExpression of human p53 mutant R248Q in mammalian cellsDepositorInsertp53(R248Q) (TP53 Human)
UseTagsExpressionMammalianMutationR248QPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BLRR
Plasmid#158958PurposeBioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).DepositorInsertBLRR
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-3myc-6His-EZH2 21D
Plasmid#42664DepositorInsertEZH2 (EZH2 Human)
UseTags3Myc and 6HisExpressionMammalianMutationS21DPromoterAvailable sinceJuly 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
ADAMTS5_pCSdest
Plasmid#53819DepositorInsertADAMTS5 (ADAMTS5 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP
Plasmid#90015PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFPDepositorInsertsCBirA*-Ago2
NBirA*-GFP
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-3myc-6His-EZH2 21A
Plasmid#42663DepositorInsertEZH2 (EZH2 Human)
UseTags3Myc and 6HisExpressionMammalianMutationS21APromoterAvailable sinceJuly 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn A30P
Plasmid#105726PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein A30P (SNCA Human)
UseTagsExpressionBacterialMutationA30PPromoterT7Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGMCtN-TSC10M1-sspB
Plasmid#49521PurposeNourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety siteDepositorInsertTSC10M1-sspB
UseTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
NSK026 CMV-TO-p53R248W (FLP-IN)
Plasmid#191168PurposeExpression of human p53 mutant R248W in mammalian cellsDepositorInsertp53(R248W) (TP53 Human)
UseTagsExpressionMammalianMutationR248WPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only