We narrowed to 1,801 results for: nif

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pVC_CRISPR_Cat

Plasmid

#120368

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR_Cat

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pKEW212-MBP-TEV-AcrVA4

Plasmid

#115658

Purpose

Expresses AcrVA4 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCJH019_SC101_pLAC_Cas12c(4)_Kan

Plasmid

#183071

Purpose

Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBA787

Plasmid

#186242

Purpose

soc-C HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH021_SC101_pLAC_Cas12c2_Kan

Plasmid

#183072

Purpose

Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c2

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH059_proD_Cas12c_DualRNA-GFP_CAM

Plasmid

#183076

Purpose

Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and pre-crRNA

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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JAB2121

Plasmid

#190343

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Type

Empty backbone

Expression

Plant

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBA789

Plasmid

#186244

Purpose

soc-F HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBA790

Plasmid

#186245

Purpose

dnap-C HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

F13A

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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L0-I18 Ub-Arg GG1-GG2

Plasmid

#195899

Purpose

Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Ub-Arg tag

Use

Synthetic Biology

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCF823_EFS-Cas9-PuroR_lenti

Plasmid

#138317

Purpose

Lenti expression of SpyCas9 with Puro selection

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBA788

Plasmid

#186243

Purpose

soc-S HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAW206

Plasmid

#113246

Purpose

E. coli expression vector for IHFa K15A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K15A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

K15A

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#113234

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->CCATC

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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L1P63

Plasmid

#195938

Purpose

PhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35S

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-PI-tUBQ1-p35S-IP-t35S

Expression

Plant

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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L0-I2 pPP2AA3-mutated GG0-GG1

Plasmid

#195891

Purpose

Level 0 pPP2AA3 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3

Use

Synthetic Biology

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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L0-T1 Target PhiC31

Plasmid

#195890

Purpose

Level 0 PhiC31 target: attP_PhiC31-pUBQ10-attB_PhiC31 with golden gate linker in pBLUNT plasmid.

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 Target

Use

Synthetic Biology

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I38 BpiI-pUBQ10-BpiI

Plasmid

#195888

Purpose

Level 0 pUBQ10 promoter between BpiI sites for target construction

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L1P5

Plasmid

#195920

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pPP2AA3-PhiC31-NLS-DST-tUBQ1

Expression

Plant

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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