We narrowed to 2,148 results for: nif

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p2CT-His-MBP-Lne_Cas13a_WT

Plasmid

#91866

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LneCas13a (LneC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Rca_Cas13a_WT

Plasmid

#91870

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

RcaCas13a (RcaC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSHS293

Plasmid

#87348

Purpose

D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/D435C/C574S/E945C

Promoter

T7

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Cam_Cas13a_WT

Plasmid

#91869

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

CamCas13a (CamC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Ere_Cas13a_WT

Plasmid

#91868

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

EreCas13a (EreC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSHS248

Plasmid

#87354

Purpose

S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S/S867C/N1054C

Promoter

T7

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

Plasmid

#39300

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S16A, R17A, H18A, D19A, W23A and N25A

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
7xNLS-pMJ915v2-sfGFP

Plasmid

#88922

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGP4G-TPLRD1-ccdB

Plasmid

#83877

Purpose

Destination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assays

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-AFB2

Plasmid

#83845

Purpose

Gateway donor vector for AFB2

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

AFB2 (AFB2 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2-sfGFP

Plasmid

#88920

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pUPD-MTS2 (12)

Plasmid

#101698

Purpose

MTS2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA7

Plasmid

#83871

Purpose

Gateway donor vector for IAA7

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA7 (IAA7 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA8

Plasmid

#83873

Purpose

Gateway donor vector for IAA8

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA8 (IAA8 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA31

Plasmid

#83864

Purpose

Gateway donor vector for IAA31

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA31 (IAA31 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA16

Plasmid

#83854

Purpose

Gateway donor vector for IAA16

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA16 (IAA16 Mustard Weed)

Use

Gateway donor vector

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)

Plasmid

#39306

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E211A, K212A, E213A, R214A, D215A and Y217A

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
CL20_mEGFP_S100A9_KRT4

Plasmid

#205916

Purpose

Express mEGFP-tagged fusion protein, S100A9_KRT4 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

S100A9_KRT4 (S100A9, KRT4 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Article

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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